Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 1714160..1714957 Replicon chromosome
Accession NZ_CP095165
Organism Enterobacter roggenkampii strain K475-2

Toxin (Protein)


Gene name KacT Uniprot ID A0A155TD66
Locus tag MUY30_RS08075 Protein ID WP_032651255.1
Coordinates 1714436..1714957 (+) Length 174 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID W7PG43
Locus tag MUY30_RS08070 Protein ID WP_008499829.1
Coordinates 1714160..1714429 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MUY30_RS08040 (MUY30_08040) 1709782..1710225 - 444 WP_008499834.1 helix-turn-helix domain-containing protein -
MUY30_RS08045 (MUY30_08045) 1710378..1711619 + 1242 WP_008499833.1 bifunctional glucose-1-phosphatase/inositol phosphatase -
MUY30_RS08050 (MUY30_08050) 1711656..1711883 - 228 WP_008499832.1 YccJ family protein -
MUY30_RS08055 (MUY30_08055) 1711904..1712500 - 597 WP_008499831.1 NAD(P)H:quinone oxidoreductase -
MUY30_RS08060 (MUY30_08060) 1712892..1713062 + 171 WP_013097201.1 general stress protein -
MUY30_RS08065 (MUY30_08065) 1713181..1714098 + 918 WP_023292825.1 DMT family transporter -
MUY30_RS08070 (MUY30_08070) 1714160..1714429 + 270 WP_008499829.1 DUF1778 domain-containing protein Antitoxin
MUY30_RS08075 (MUY30_08075) 1714436..1714957 + 522 WP_032651255.1 GNAT family N-acetyltransferase Toxin
MUY30_RS08080 (MUY30_08080) 1715031..1716353 - 1323 WP_045352935.1 pyrimidine utilization transport protein G -
MUY30_RS08085 (MUY30_08085) 1716375..1716869 - 495 WP_023327078.1 pyrimidine utilization flavin reductase protein F -
MUY30_RS08090 (MUY30_08090) 1716879..1717469 - 591 WP_126287632.1 malonic semialdehyde reductase -
MUY30_RS08095 (MUY30_08095) 1717479..1718279 - 801 WP_021240807.1 pyrimidine utilization protein D -
MUY30_RS08100 (MUY30_08100) 1718287..1718673 - 387 WP_008499822.1 pyrimidine utilization protein C -
MUY30_RS08105 (MUY30_08105) 1718685..1719374 - 690 WP_047745127.1 pyrimidine utilization protein B -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 174 a.a.        Molecular weight: 19714.46 Da        Isoelectric Point: 7.0497

>T241632 WP_032651255.1 NZ_CP095165:1714436-1714957 [Enterobacter roggenkampii]
VDNLTIEMFSEGKDYDFRDFDCGEPSLNVFLTEHLVRQHNGRILRGYLLKDRDGPRVLGYYTLSGSCFEKAMLPSKTQQR
RIPYSNVPSVTLGRLAIHKDLQGLEWGTTLVTHAMRVVYLASQAVGVHGIFVDALNDQAKQFYLKQGFIPLTDENSHSLF
FPTKSIERLFEQA

Download         Length: 522 bp

>T241632 NZ_LT996886:1634940-1635338 [Tessaracoccus timonensis]
ATGGTGACGTACTTGCTCGATACGAGCATCTGGATTGAGCTCCTGCGAGGGAACGAATCGGTCGTACAACGGATGCAGCG
CTGCTCACCTCGGGACATCAGATTATCTCCCATCGTCCAGGGGGAGTTGGAGACGGGGCGGCAGCTGTCGCAGTCCGCTG
ACCAAGCGGAGATGCTGGAGTGGGTGTATCACTCCTTTCCCCGCAACGCCTTCGGGGATACCGTGGCGCGTCAGTGTGGT
GTCGTGCGCGCCCAGCTCCGCCGCGCAGGTACGCCAATCGGGGTGAACGACACCTGGATCGCCGCGGAAGCCCTCGCGCA
CGGGCAGACCGTCGTTACCGCGAACGAGCGTGATTTCCGTCGCGTGCCGGGGCTCAAGGTCGAAAACTGGATGGTTTGA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10262.72 Da        Isoelectric Point: 5.8836

>AT241632 WP_008499829.1 NZ_CP095165:1714160-1714429 [Enterobacter roggenkampii]
MPALKKQRIDLRLTDEDKTMIEEAAAMTNQTITQFMVNSASERAAEVIEQHRRLVLNEASWNAVMDAIDNPPEPNERLKR
AAKRLRNME

Download         Length: 270 bp

>AT241632 NZ_LT996886:1634704-1634946 [Tessaracoccus timonensis]
ATGACGCCCCGCACCACCACCGTCTTCAAATCTGGCAACTCGCAGGCCGTGCGCATCCCGAAGGACCTAGCGTTTTCGAC
GGACAAGGTCACTATTGAAGTCGACGGCGACGCGCTCATCTTGCGCCCCCAGCCCCTCACCATGCAGGAATTCCTCGACG
GTCTGCCGCGGCACCCAGGAGCCTTCGAGAACTTTCCGGACTTCGAGGACGAGATTGATGAGGAGCCGGTGCCGCAATGG
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A155TD66

Antitoxin

Source ID Structure
AlphaFold DB W7PG43

References