Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1128966..1129586 Replicon chromosome
Accession NZ_CP095165
Organism Enterobacter roggenkampii strain K475-2

Toxin (Protein)


Gene name Hha Uniprot ID A0A1H8SI38
Locus tag MUY30_RS05310 Protein ID WP_008499287.1
Coordinates 1128966..1129184 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID V3PBI9
Locus tag MUY30_RS05315 Protein ID WP_008499288.1
Coordinates 1129212..1129586 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MUY30_RS05280 (MUY30_05280) 1124979..1125239 + 261 WP_006176933.1 type B 50S ribosomal protein L31 -
MUY30_RS05285 (MUY30_05285) 1125242..1125382 + 141 WP_003859006.1 type B 50S ribosomal protein L36 -
MUY30_RS05290 (MUY30_05290) 1125379..1126089 - 711 WP_126288742.1 GNAT family protein -
MUY30_RS05295 (MUY30_05295) 1126191..1127651 + 1461 WP_126288744.1 PLP-dependent aminotransferase family protein -
MUY30_RS05300 (MUY30_05300) 1127623..1128090 - 468 WP_008499285.1 YlaC family protein -
MUY30_RS05305 (MUY30_05305) 1128208..1128759 - 552 WP_008499286.1 maltose O-acetyltransferase -
MUY30_RS05310 (MUY30_05310) 1128966..1129184 - 219 WP_008499287.1 HHA domain-containing protein Toxin
MUY30_RS05315 (MUY30_05315) 1129212..1129586 - 375 WP_008499288.1 Hha toxicity modulator TomB Antitoxin
MUY30_RS05320 (MUY30_05320) 1130096..1133242 - 3147 WP_008499289.1 multidrug efflux RND transporter permease subunit AcrB -
MUY30_RS05325 (MUY30_05325) 1133265..1134458 - 1194 WP_021241633.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8612.00 Da        Isoelectric Point: 8.9008

>T241631 WP_008499287.1 NZ_CP095165:c1129184-1128966 [Enterobacter roggenkampii]
MSDKPLTKIDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFVR

Download         Length: 219 bp

>T241631 NZ_LT996886:791907-792338 [Tessaracoccus timonensis]
ATGCTGCTCGTAGATACCAATGTGTTGATCTACGCAGTCAACGCCAGCTCCGCTCATCACGACGCCAGCCGCACTTGGTT
TTTGCATGAGCTGCAATCAGGCACGGACGTGGTCGGCTTGCCGTGGGTGTCTCTGCTCGGGTTCATCCGCATCAGCACTC
ACTCGCACATCCTGGAGCACCCACTCTCGCCCGAGGAAGCGATGTCGGTGGTACACGCATGGCTCAATCACCCGCGAGTG
GTAACACCTGAGCCTTCGCCCCGGCACGCTGCCCTGCTTGCGGGGCTTCTGGCGGAATCCGGCACGGCCGGCAATCTCAC
CAACGATGCTCACCTGGCTGCCCTGGCGCTGGAACTCGATGCGACGATGGTCACCTTTGATCGCGACTTCGCTCGCTTCG
GAGTTCGCGTGCTCGTTCCGGAACACGAGTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14481.28 Da        Isoelectric Point: 4.8886

>AT241631 WP_008499288.1 NZ_CP095165:c1129586-1129212 [Enterobacter roggenkampii]
MDEYSPKRHDIAQLKFLCESLYHDCLANLDESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFVNVSRANPVSLSC

Download         Length: 375 bp

>AT241631 NZ_LT996886:791656-791907 [Tessaracoccus timonensis]
ATGCGAACGACGCTCACCCTCGACCCCGATGTAGCCAAGCTGCTGGAGGAAGAGATGGCCAAACGTCAGACTTCATTCAA
AAGAGTGGTGAACGACGCCCTACGTCGAGGCCTCGGATCACACCATGACGCCGACTTCACCTTCCCCAGCTTCGACATGG
GATCAGCTAACGCAGATCTCACCCATGCCACCAGGCTCGCTGCCGACATGGAAGACGAAGCCCTCGCATTCAAGCTCACC
CAAGGCCGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1H8SI38

Antitoxin

Source ID Structure
AlphaFold DB V3PBI9

References