Detailed information of TA system TA241627

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-MazE
• Location: 48267..48928
• Replicon: chromosome
• Accession: NZ_CP095165
• Organism: Enterobacter roggenkampii strain K475-2

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: MUY30_RS00245
• Protein ID: WP_023293716.1
• Coordinates: 48530..48928 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A443U1A0
• Locus tag: MUY30_RS00240
• Protein ID: WP_032654366.1
• Coordinates: 48267..48533 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MUY30_RS00215 (MUY30_00215)	44331..45659	-	1329	WP_032675701.1	PTS sugar transporter subunit IIC	-
MUY30_RS00220 (MUY30_00220)	45671..45985	-	315	WP_021242703.1	PTS sugar transporter subunit IIB	-
MUY30_RS00225 (MUY30_00225)	46279..47229	+	951	WP_126288210.1	LacI family DNA-binding transcriptional regulator	-
MUY30_RS00230 (MUY30_00230)	47280..47576	-	297	WP_032654360.1	YicS family protein	-
MUY30_RS00235 (MUY30_00235)	47718..48137	+	420	WP_032654363.1	GNAT family N-acetyltransferase	-
MUY30_RS00240 (MUY30_00240)	48267..48533	+	267	WP_032654366.1	virulence protein	Antitoxin
MUY30_RS00245 (MUY30_00245)	48530..48928	+	399	WP_023293716.1	type II toxin-antitoxin system VapC family toxin	Toxin
MUY30_RS00250 (MUY30_00250)	49068..49868	+	801	WP_126288208.1	lipoprotein NlpA	-
MUY30_RS00255 (MUY30_00255)	49992..50771	+	780	WP_032654371.1	MBL fold metallo-hydrolase	-
MUY30_RS00260 (MUY30_00260)	50798..51751	+	954	WP_126288206.1	helix-turn-helix domain-containing protein	-
MUY30_RS00265 (MUY30_00265)	51934..53631	+	1698	WP_241236508.1	ShlB/FhaC/HecB family hemolysin secretion/activation protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-121)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14799.00 Da
• Isoelectric Point: 6.4779

>T241627 WP_023293716.1 NZ_CP095165:48530-48928 [Enterobacter roggenkampii]
MRHMLDTNIVSHLFKRHPEVVSRMTCLAPGDVCISSITEAELLYGADKKKSSRLKETIREFLNTITICDWDSDAAATYGE
LRATLEKKGKVMGDLDQLIAAHAISRGTTIVTNDRAFRMVQELAVEDWTTTL

Nucleotide

• Length: 399 bp

>T241627 NZ_LT996885:1401530-1401805 [Dialister massiliensis]
ATGAAGTACGAAGTAAAATTCACCACTCAATTTAAGAAAGATTTGAAATTGGCAAAGAAGCAGAACAAAGATATAGATGT
GCTGTTCTCTGTCATTGAGCAATTGGCCCAAGGAAAACAATTGGATGAAAAATATAGAGACCATGATTTAGGTGGAACAT
ACAAAGGTTGCCGGGAATGCCATATTGATCCAGATTGGCTTCTCATTTATGAAACCAAAGATGATGTACTTGTTCTTCTG
CTATATCGTTTGGGCAGTCATTCCCAATTATTTTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10009.38 Da
• Isoelectric Point: 4.6310

>AT241627 WP_032654366.1 NZ_CP095165:48267-48533 [Enterobacter roggenkampii]
MERTAKLFKKGQNQAVMLPAEFAFDTESVYIRRDEEGNVVLVAKPVLIKSWDNFLRMLAETDVPDTFLSKEERNQSVTTR
DPLDGACS

Nucleotide

• Length: 267 bp

>AT241627 NZ_LT996885:1401261-1401533 [Dialister massiliensis]
ATGGTAAATACAAATTTGAATATCCGGACGGATAAGGAAGTCAAAAATCAGGCTGAGAAAATATTCAATGCTCTGGGAAT
GAATATGACGACGGCGGTAAACATATTCTTAAAAACAACGATACGAGAAAATGGCATTCCCTTCCGTCTCACTCTTGACG
TTCCTAATGCAACAACTAGATCTGCCATTGAAGAAGGCAAACGAATCGCCATTGATAAAAAAGTAAAAGGGTATACCAAT
ATGACAGATTTGCGTGTGGCCCTTGAAAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A443U1A0