241596

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 150923..151448 Replicon plasmid pIso00041_01
Accession NZ_CP095156
Organism Escherichia coli strain Iso00041

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag MU410_RS24905 Protein ID WP_001159871.1
Coordinates 150923..151228 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID H9TJP1
Locus tag MU410_RS24910 Protein ID WP_000813630.1
Coordinates 151230..151448 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MU410_RS24890 (146885) 146885..148051 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
MU410_RS24895 (148639) 148639..149394 - 756 WP_000852146.1 replication initiation protein RepE -
MU410_RS24900 (150116) 150116..150922 - 807 WP_000016970.1 site-specific integrase -
MU410_RS24905 (150923) 150923..151228 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
MU410_RS24910 (151230) 151230..151448 - 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
MU410_RS24915 (152008) 152008..152238 + 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin -
MU410_RS24920 (152235) 152235..152651 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -
MU410_RS24925 (152726) 152726..154291 + 1566 WP_001128474.1 AAA family ATPase -
MU410_RS24930 (154276) 154276..155298 + 1023 WP_000361402.1 helicase UvrD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaOXA-1 / aac(6')-Ib-cr / blaCTX-M-15 / tet(B) / catA1 / mph(A) / sul1 / qacE / aadA5 / aac(3)-IId / blaTEM-1B / sitABCD iutA / iucD / iucC / iucB / iucA 1..174508 174508
- flank IS/Tn - - 155552..156055 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T241596 WP_001159871.1 NZ_CP095156:c151228-150923 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T241596 NZ_LT992491:912834-913124 [Vibrio cholerae]
ATGACCTATAAGCTTGAGTTCAAAAAGAGTGCTTTGAAGGAATGGAAAAAGTTAGCCGTTCCACTCCAACAGCAGTTCAA
GAAGAAACTAATAGAACGCCTTGAAAATCCACATGTTCCATCAGCCAAACTGTCTGGCGCTGAGAACATTTATAAAATAA
AACTTCGGCAGTCTGGTTATCGCTTAGTCTATCAAGTTGAAAATGACATCATCGTAGTTACTGTTTTAGCTGTCGGTAAA
CGAGAACGCAGCGAAGTTTACACTAAAGCGCTGCAACGGCTAGACGACTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8354.27 Da        Isoelectric Point: 4.5203

>AT241596 WP_000813630.1 NZ_CP095156:c151448-151230 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT241596 NZ_LT992491:912596-912844 [Vibrio cholerae]
ATGACCACTAGAATCCTAGCTGATGTTGCAGCAAGCATTACCGAATTTAAAGCAAACCCAATGAAAGTGGCAACTAGCGC
CTTCGGTGCTCCTGTTGCTGTACTCAACCGAAATGAGCCGGCTTTCTACTGCGTCCCTGCAAGCACCTACGAGATCATGA
TGGATAAGCTTGAAGATCTTGAGCTACTGGCTATTGCCAAAAAACGCTTAAGTGAAGACAGCGTATCGGTAAATATCGAT
GACCTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CEF5

References