Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4490756..4491253 Replicon chromosome
Accession NZ_CP095155
Organism Escherichia coli strain Iso00041

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag MU410_RS21765 Protein ID WP_001173343.1
Coordinates 4490756..4490929 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag MU410_RS21770 Protein ID WP_000398619.1
Coordinates 4490957..4491253 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MU410_RS21755 (4487426) 4487426..4488883 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
MU410_RS21760 (4489120) 4489120..4490637 + 1518 WP_001295074.1 lysine--tRNA ligase -
MU410_RS21765 (4490756) 4490756..4490929 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
MU410_RS21770 (4490957) 4490957..4491253 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
MU410_RS21775 (4491480) 4491480..4491752 - 273 WP_000405651.1 GNAT family N-acetyltransferase -
MU410_RS21780 (4491764) 4491764..4491994 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
MU410_RS21785 (4492175) 4492175..4493806 + 1632 WP_001216477.1 sensor histidine kinase -
MU410_RS21790 (4493803) 4493803..4494522 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T241588 WP_001173343.1 NZ_CP095155:c4490929-4490756 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T241588 NZ_LT992491:829890-830387 [Vibrio cholerae]
ATGATGAATACGGTACTTCTCGATAAAGATAAGCACGATAGGAACCGTTTCAATTGTGGCACTGAAGCCCTCAACAATTA
CTTAAAAGTAATGGCGAGTCAGCAAGCCAAAAAAGACAACACCAGAACCTTCGTTTTGGAAGATGACAATAACAGTGCTT
ATATCATTGGCTTTTACACTCTGACTATGACGCCTATTGATTTGAAAGCCTTGCCCGACAAGTTACAAAAGAAACACCAA
TCCTCAACCTCTGGAGGGCTCATTGCTCGTTTAGCTGTCGACGATAGATACAAAGGAAAAGGTTTTGGCGAGTGGCTACT
CATTGACGCTCTCAGAAAGCTACTCGCAGCCAGTGACAGCGTAGCGTTCCCAGTTGTCATTGTTGATGCCAAAGATGGTG
CAAAACATTTCTATGAGCGCTATGGGTTTCAAGCGTTTCAAGATGCCGAAAACAAACTCTTCATCACCATTGCTGATATC
AGAGCAGGCTTAGGCTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT241588 WP_000398619.1 NZ_CP095155:c4491253-4490957 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT241588 NZ_LT992491:829621-829893 [Vibrio cholerae]
ATGGCAACGACTTTGCCTCGCATCACCGCTAGAGTTGATGTCGATACTCAAGATTTACTCACTAAGGCTGCTGCTCTTGC
TGGCATGTCTAGTATTAACTCATTTGTTTTGAATGCGGCAATCGAAAAAGCAAAACAAGTCATTGAACGTGAGCAAGCTC
TAAAGCTTAGCCAAGTTGATGCAGTATTGCTGATGGAAGCTCTTGATAATCCAGCGGTAGCAAATGCAAAACTCAAATTG
GCATCCGAACGTTATGAGAGCAAAACTCAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References