Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5345149..5345774 Replicon chromosome
Accession NZ_CP095150
Organism Klebsiella pneumoniae strain Iso00073

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag MU411_RS25990 Protein ID WP_002882817.1
Coordinates 5345149..5345532 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag MU411_RS25995 Protein ID WP_004150355.1
Coordinates 5345532..5345774 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MU411_RS25975 5342515..5343417 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
MU411_RS25980 5343414..5344049 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
MU411_RS25985 5344046..5344975 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
MU411_RS25990 5345149..5345532 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
MU411_RS25995 5345532..5345774 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
MU411_RS26000 5345979..5346896 + 918 WP_004187175.1 alpha/beta hydrolase -
MU411_RS26005 5346910..5347851 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
MU411_RS26010 5347896..5348333 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
MU411_RS26015 5348330..5349190 - 861 WP_019725422.1 virulence factor BrkB family protein -
MU411_RS26020 5349184..5349783 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T241557 WP_002882817.1 NZ_CP095150:c5345532-5345149 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T241557 NZ_LT992487:933707-933997 [Vibrio cholerae]
ATGACCTATAAGCTTGAGTTCAAAAAGAGTGCTTTGAAGGAATGGAAAAAGTTAGCCGTTCCACTCCAACAGCAGTTCAA
GAAGAAACTAATAGAACGCCTTGAAAATCCACATGTTCCATCAGCCAAACTGTCTGGCGCTGAGAACATTTATAAAATAA
AACTTCGGCAGTCTGGTTATCGCTTAGTCTATCAAGTTGAAAATGACATCATCGTAGTTACTGTTTTAGCTGTCGGTAAA
CGAGAACGCAGCGAAGTTTACACTAAAGCGCTGCAACGGCTAGACGACTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT241557 WP_004150355.1 NZ_CP095150:c5345774-5345532 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT241557 NZ_LT992487:933469-933717 [Vibrio cholerae]
ATGACCACTAGAATCCTAGCTGATGTTGCAGCAAGCATTACCGAATTTAAAGCAAACCCAATGAAAGTTGCAACTAGTGC
CTTCGGTGCTCCTGTTGCTGTACTCAACCGAAATGAGCCGGCTTTCTACTGCGTCCCTGCAAGCACCTACGAGATCATGA
TGGATAAGCTTGAAGATCTTGAGCTACTGGCTATTGCCAAAGAACGCTTAAGTGAAGACAGCGTATCGGTAAATATCGAT
GACCTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References