Detailed information of TA system TA241539

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 39960..40485
• Replicon: plasmid pIso00100_02
• Accession: NZ_CP095147
• Organism: Klebsiella pneumoniae strain Iso00100

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: MU412_RS27125
• Protein ID: WP_012539978.1
• Coordinates: 40180..40485 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: R4WC25
• Locus tag: MU412_RS27120
• Protein ID: WP_015632547.1
• Coordinates: 39960..40178 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MU412_RS27100	35205..36707	+	1503	WP_048296350.1	RES family NAD+ phosphorylase	-
MU412_RS27105	36900..37250	-	351	WP_015632544.1	hypothetical protein	-
MU412_RS27110	37828..38013	+	186	WP_032433930.1	hypothetical protein	-
MU412_RS27115	38086..39360	-	1275	WP_015632546.1	hypothetical protein	-
MU412_RS27120	39960..40178	+	219	WP_015632547.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
MU412_RS27125	40180..40485	+	306	WP_012539978.1	type II toxin-antitoxin system toxin CcdB	Toxin
MU412_RS27130	40672..41676	+	1005	WP_029497484.1	hypothetical protein	-
MU412_RS27135	41874..42653	+	780	WP_015632549.1	site-specific integrase	-
MU412_RS27140	42711..42968	-	258	WP_032433931.1	hypothetical protein	-
MU412_RS27145	43096..43209	-	114	WP_032072094.1	hypothetical protein	-
MU412_RS27150	43841..44596	+	756	WP_012539983.1	replication initiation protein RepE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	mph(A) / aph(3')-Ia / sul1 / qnrB6 / aadA16 / dfrA27 / ARR-3 / aac(6')-Ib-cr / sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1B / blaCTX-M-15 / blaOXA-1 / aac(3)-IIa	-	1..109388	109388

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11601.27 Da
• Isoelectric Point: 6.4661

>T241539 WP_012539978.1 NZ_CP095147:40180-40485 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDDSYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T241539 NZ_LT992477:1359480-1359941 [Staphylococcus aureus]
ATGGGATTATATGAAGAAACTTTAATACAACATGATTATATTGAAACAAGAGAGGCTGATGTGCTTCCAGATAATTTGGA
TGGGGTATGGTTAGGAGATTTAATTTTAATAAAGCGTGGCTTATCAGATAGAGAAAAGGCAGGAATTCTCTTTGAAGAAT
TAGCACATAATAAACTTACATACGGTGATATAGCCGATTACTCGAAATTCAACAATCGCAAGTTCGAAAATTATGCACGT
AGACATGGTTTTACTTCAGCTGTACCGCTACATGAAATTGTAGAAGCTCACAACTACGGTGTTCGTAATTTGTACGAGTT
GTCAGAGTATCTGCAGTTGAGTGAATCATACATACTAGAAGCTATAGAACAATATAAAAAGATATATGGTATTGGAACTC
ACTATGGCGAGTATTCTATTACGTTTGAGCCGTTGAGAGTTTTTAAATATAAGGAAATATAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8362.31 Da
• Isoelectric Point: 4.7247

>AT241539 WP_015632547.1 NZ_CP095147:39960-40178 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRAERWQAENQEGMAEVARFIDMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT241539 NZ_LT992477:1359144-1359467 [Staphylococcus aureus]
ATGAGAACTTCAGCGGAAATAGGTAAATTAATCAAACAACTACGAAAAGAGAATAATGTGAATTTAACTGATTTTGCAAC
TAAGATAGGTGTCAATAAATCTACCTTATCCCGATATGAAAACGGTAGCAGAAAAATACCTATGGAGGATATAGCTGAGA
TTGCCAATGCATTGAAAGTTACCCCAGAATATTTACTATTAAAAAATAGACAAACAGAAAACGAAGTACAACATCGTGCA
GCTCACCTTGAAGGAGAATTGACAGATGATGAATGGCAAAGAGTTTTAGATTATGCAGATTATATAAGAAGCAAACGTAA
GTAA

Structures

Antitoxin

• AlphaFold DB: A0A2L1KT86