Detailed information of TA system TA241538

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 6195..6838
• Replicon: plasmid pIso00100_02
• Accession: NZ_CP095147
• Organism: Klebsiella pneumoniae strain Iso00100

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: J5XEP2
• Locus tag: MU412_RS26940
• Protein ID: WP_009653152.1
• Coordinates: 6422..6838 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q84A07
• Locus tag: MU412_RS26935
• Protein ID: WP_001261276.1
• Coordinates: 6195..6425 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MU412_RS26905	1486..2112	+	627	WP_016946792.1	ParA family plasmid-partitioning AAA ATPase	-
MU412_RS26910	2109..2411	+	303	WP_004197636.1	hypothetical protein	-
MU412_RS26915	2867..3661	-	795	WP_065801584.1	site-specific integrase	-
MU412_RS26920	3821..4876	-	1056	WP_225322422.1	hypothetical protein	-
MU412_RS26925	5070..5375	-	306	WP_006788214.1	type II toxin-antitoxin system toxin CcdB	-
MU412_RS26930	5377..5595	-	219	WP_015632547.1	type II toxin-antitoxin system antitoxin CcdA	-
MU412_RS26935	6195..6425	+	231	WP_001261276.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
MU412_RS26940	6422..6838	+	417	WP_009653152.1	type II toxin-antitoxin system VapC family toxin	Toxin
MU412_RS26945	7082..7938	+	857	Protein_9	IS3-like element ISEc15 family transposase	-
MU412_RS26950	8220..8567	+	348	Protein_10	transposase	-
MU412_RS26955	8630..9334	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
MU412_RS26960	9387..9527	-	141	Protein_12	IS6 family transposase	-
MU412_RS26965	9591..9755	+	165	Protein_13	helix-turn-helix transcriptional regulator	-
MU412_RS26970	9799..9864	+	66	Protein_14	EAL domain-containing protein	-
MU412_RS26975	9982..10746	+	765	WP_001389365.1	IS6-like element IS6100 family transposase	-
MU412_RS26980	11239..11823	-	585	WP_001137892.1	macrolide-binding transcriptional repressor MphR(A)	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	mph(A) / aph(3')-Ia / sul1 / qnrB6 / aadA16 / dfrA27 / ARR-3 / aac(6')-Ib-cr / sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1B / blaCTX-M-15 / blaOXA-1 / aac(3)-IIa	-	1..109388	109388

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15048.60 Da
• Isoelectric Point: 9.2957

>T241538 WP_009653152.1 NZ_CP095147:6422-6838 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQVVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFARVPGLVLEDWVK

Nucleotide

• Length: 417 bp

>T241538 NZ_LT992477:799984-800172 [Staphylococcus aureus]
ATGTCTTTACATTTTGCAATTCTGTTTTGGCTAGCATTAATTTTCTTAGTTGCCGCTACGTTTATACTCGTATTAATGAA
AAAAACTGGCAAAGAATCTAAAAAAGAGTCCTATTTAAGTTTCACTGTCATTCTCTATATTTTTGGATTCGCTATATTAA
TATACACATTTATATTTGGTGTGCTATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8721.74 Da
• Isoelectric Point: 4.3533

>AT241538 WP_001261276.1 NZ_CP095147:6195-6425 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT241538 NZ_LT992477:799696-799956 [Staphylococcus aureus]
ATGAATAACATTTTGTTAAATGCTATCAATATAGTTATTACTACCACTTTTGTTATCTTTAATATTTTAATCACATATAA
TAAAGATTTAGATGATTTATGTTGGCTCCTGCCTGGTATTATCATTTGTGGTGTGATACTCATAGTATCCTTTACCATTG
CAATGATAACTAAAAACTGGTTAAGTGAAATATTATTTTTTATAAATATCGTACTCGTTCTCTATTACATTTATCCTATT
TTTTATAGTTTTATAGGTTAA

Structures

Toxin

• AlphaFold DB: A0A486V9L7

Antitoxin

• AlphaFold DB: A0A387K023