Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 5070..5595 Replicon plasmid pIso00100_02
Accession NZ_CP095147
Organism Klebsiella pneumoniae strain Iso00100

Toxin (Protein)


Gene name ccdB Uniprot ID A0A1D8K3Q5
Locus tag MU412_RS26925 Protein ID WP_006788214.1
Coordinates 5070..5375 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID R4WC25
Locus tag MU412_RS26930 Protein ID WP_015632547.1
Coordinates 5377..5595 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MU412_RS26905 1486..2112 + 627 WP_016946792.1 ParA family plasmid-partitioning AAA ATPase -
MU412_RS26910 2109..2411 + 303 WP_004197636.1 hypothetical protein -
MU412_RS26915 2867..3661 - 795 WP_065801584.1 site-specific integrase -
MU412_RS26920 3821..4876 - 1056 WP_225322422.1 hypothetical protein -
MU412_RS26925 5070..5375 - 306 WP_006788214.1 type II toxin-antitoxin system toxin CcdB Toxin
MU412_RS26930 5377..5595 - 219 WP_015632547.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
MU412_RS26935 6195..6425 + 231 WP_001261276.1 type II toxin-antitoxin system VapB family antitoxin -
MU412_RS26940 6422..6838 + 417 WP_009653152.1 type II toxin-antitoxin system VapC family toxin -
MU412_RS26945 7082..7938 + 857 Protein_9 IS3-like element ISEc15 family transposase -
MU412_RS26950 8220..8567 + 348 Protein_10 transposase -
MU412_RS26955 8630..9334 + 705 WP_001067855.1 IS6-like element IS26 family transposase -
MU412_RS26960 9387..9527 - 141 Protein_12 IS6 family transposase -
MU412_RS26965 9591..9755 + 165 Protein_13 helix-turn-helix transcriptional regulator -
MU412_RS26970 9799..9864 + 66 Protein_14 EAL domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid mph(A) / aph(3')-Ia / sul1 / qnrB6 / aadA16 / dfrA27 / ARR-3 / aac(6')-Ib-cr / sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1B / blaCTX-M-15 / blaOXA-1 / aac(3)-IIa - 1..109388 109388


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11687.46 Da        Isoelectric Point: 7.2015

>T241537 WP_006788214.1 NZ_CP095147:c5375-5070 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRLMTTDMASVPASVTGEEV
ADLRHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T241537 NZ_LT992477:c641339-641232 [Staphylococcus aureus]
GTGATATCTATTGCAAATGCATTACATTTAATGTTAAGTTTCGGTATGTTTATCGTCACTTTCATTGGTGTAGTAGTCGC
AATAATTAATTTAAACAATAAAAAATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8362.31 Da        Isoelectric Point: 4.7247

>AT241537 WP_015632547.1 NZ_CP095147:c5595-5377 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRAERWQAENQEGMAEVARFIDMNGSFADENRDW

Download         Length: 219 bp

>AT241537 NZ_LT992477:641142-641179 [Staphylococcus aureus]
AACATGTCGCCTAATGAGCCCGTTAAAAAGACGGTGAC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1D8K3Q5

Antitoxin

Source ID Structure
AlphaFold DB A0A2L1KT86

References