Detailed information of TA system TA241535

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 5186898..5187523
• Replicon: chromosome
• Accession: NZ_CP095145
• Organism: Klebsiella pneumoniae strain Iso00100

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: R4Y4A3
• Locus tag: MU412_RS25285
• Protein ID: WP_002882817.1
• Coordinates: 5186898..5187281 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: MU412_RS25290
• Protein ID: WP_162557435.1
• Coordinates: 5187281..5187523 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MU412_RS25270 (5184264)	5184264..5185166	+	903	WP_002882822.1	formate dehydrogenase subunit beta	-
MU412_RS25275 (5185163)	5185163..5185798	+	636	WP_002882818.1	formate dehydrogenase cytochrome b556 subunit	-
MU412_RS25280 (5185795)	5185795..5186724	+	930	WP_004150358.1	formate dehydrogenase accessory protein FdhE	-
MU412_RS25285 (5186898)	5186898..5187281	-	384	WP_002882817.1	type II toxin-antitoxin system VapC family toxin	Toxin
MU412_RS25290 (5187281)	5187281..5187523	-	243	WP_162557435.1	CopG family transcriptional regulator	Antitoxin
MU412_RS25295 (5187728)	5187728..5188645	+	918	WP_032417884.1	alpha/beta hydrolase	-
MU412_RS25300 (5188660)	5188660..5189601	-	942	WP_004178031.1	fatty acid biosynthesis protein FabY	-
MU412_RS25305 (5189646)	5189646..5190083	-	438	WP_002882809.1	D-aminoacyl-tRNA deacylase	-
MU412_RS25310 (5190080)	5190080..5190940	-	861	WP_004146232.1	virulence factor BrkB family protein	-
MU412_RS25315 (5190934)	5190934..5191533	-	600	WP_004151865.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-112)

Antitoxin

• Ribbon-helix-helix protein, CopG (13-47)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14377.62 Da
• Isoelectric Point: 7.3178

>T241535 WP_002882817.1 NZ_CP095145:c5187281-5186898 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Nucleotide

• Length: 384 bp

>T241535 NZ_LT992476:c2735136-2734891 [Staphylococcus aureus]
ATGAACATTCAAGAAGCAACTAAGATAGCTACAAAAAATCTTGTCTCTATGACACGGAAAGATTGGAAAGAAAGTCATCG
AACTAAGATATTACCAACAAATGATAGTTTTTTACAATGCATCATTTCAAATAGCGATGGGACAAACCTTATCAGATATT
GGCAACCTTCAGCCGATGACCTCATGGCAAATGATTGGGAAGTTATAAACCCAACTAGAGACCAGGAATTATTGAAGCAA
TTTTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9557.79 Da
• Isoelectric Point: 4.3509

>AT241535 WP_162557435.1 NZ_CP095145:c5187523-5187281 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNISRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Nucleotide

• Length: 243 bp

>AT241535 NZ_LT992476:2734557-2734922 [Staphylococcus aureus]
ATGCCCTTGATATCTGATGAATTTGATACACTTACTAAAGACCAACAATATATCTTGTCCGTACTCTACAAAGATTATTT
AGAATGTGTAAAGTTAGGTTCGGTTAAATTAACCTGCAATAATTTTGGAAGTGCTAAAGATATACATACAAAGTATTTTC
AAAAACTACATTTCGAAGATGTAAAATACGATTTAAATAAACTTAAAAACTCTGGGTTCCTAAACGGCGTGTATGCTAGT
AACACTATTTATCATGTAACAATTTCAGACAAGACTGTTGTTTACTTTGAAAATGAGTTTAAAAACAATTTAAAAAGTAT
CATTGATAGCATTTCTAAAATTGCTTCAATAATTCCTGGTCTCTAG

Structures

Toxin

• AlphaFold DB: A0A0H3GPK8