Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5277751..5278376 Replicon chromosome
Accession NZ_CP095140
Organism Klebsiella pneumoniae strain Iso00199

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag MU413_RS26080 Protein ID WP_002882817.1
Coordinates 5277751..5278134 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag MU413_RS26085 Protein ID WP_004150355.1
Coordinates 5278134..5278376 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MU413_RS26065 (5275117) 5275117..5276019 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
MU413_RS26070 (5276016) 5276016..5276651 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
MU413_RS26075 (5276648) 5276648..5277577 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
MU413_RS26080 (5277751) 5277751..5278134 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
MU413_RS26085 (5278134) 5278134..5278376 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
MU413_RS26090 (5278581) 5278581..5279498 + 918 WP_023302328.1 alpha/beta hydrolase -
MU413_RS26095 (5279512) 5279512..5280453 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
MU413_RS26100 (5280498) 5280498..5280935 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
MU413_RS26105 (5280932) 5280932..5281792 - 861 WP_004146232.1 virulence factor BrkB family protein -
MU413_RS26110 (5281786) 5281786..5282385 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T241513 WP_002882817.1 NZ_CP095140:c5278134-5277751 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T241513 NZ_LT992474:c2745287-2744925 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAACAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAGCAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTAAATGCAGTAGCTCATCAGAAAAATTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT241513 WP_004150355.1 NZ_CP095140:c5278376-5278134 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT241513 NZ_LT992474:c2745454-2745284 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References