Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 4215950..4216784 Replicon chromosome
Accession NZ_CP095137
Organism Escherichia coli strain Iso00225

Toxin (Protein)


Gene name yeeV Uniprot ID A0A1J6XFW9
Locus tag MU414_RS20635 Protein ID WP_000854770.1
Coordinates 4215950..4216327 (-) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A1M1N885
Locus tag MU414_RS20640 Protein ID WP_001280950.1
Coordinates 4216416..4216784 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MU414_RS20610 (4212061) 4212061..4213683 - 1623 WP_001295726.1 Alw26I/Eco31I/Esp3I family type II restriction adenine-specific DNA-methyltransferase -
MU414_RS20615 (4214474) 4214474..4214650 - 177 Protein_4043 helix-turn-helix domain-containing protein -
MU414_RS20620 (4215017) 4215017..4215166 - 150 Protein_4044 hypothetical protein -
MU414_RS20625 (4215272) 4215272..4215448 - 177 WP_000839286.1 DUF957 domain-containing protein -
MU414_RS20630 (4215465) 4215465..4215953 - 489 WP_000761690.1 DUF5983 family protein -
MU414_RS20635 (4215950) 4215950..4216327 - 378 WP_000854770.1 TA system toxin CbtA family protein Toxin
MU414_RS20640 (4216416) 4216416..4216784 - 369 WP_001280950.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
MU414_RS20645 (4216947) 4216947..4217168 - 222 WP_001220314.1 DUF987 domain-containing protein -
MU414_RS20650 (4217231) 4217231..4217707 - 477 WP_001186779.1 RadC family protein -
MU414_RS20655 (4217723) 4217723..4218187 - 465 WP_000855061.1 antirestriction protein -
MU414_RS20660 (4218529) 4218529..4219347 - 819 WP_001234712.1 DUF932 domain-containing protein -
MU414_RS20665 (4219465) 4219465..4219660 - 196 Protein_4053 DUF905 family protein -
MU414_RS20670 (4219731) 4219731..4221632 - 1902 Protein_4054 Ag43/Cah family autotransporter adhesin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4204304..4244513 40209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14196.37 Da        Isoelectric Point: 8.7438

>T241494 WP_000854770.1 NZ_CP095137:c4216327-4215950 [Escherichia coli]
MKTLPDTHVRKVSRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDLPGF
SACTHSQLINSIDILRARRATGLMIRDNYRTVNNITLGKHPEAKR

Download         Length: 378 bp

>T241494 NZ_LT992472:c626817-626710 [Staphylococcus aureus]
ATGTTCAATTTATTAATTGACATCATGACTTCAGCTTTAAGCGGCTGTCTTGTTGCGTTTTTTGCACATTGGTTACGAAC
GCGCAACAATAAAAAAGGTGACAAATAA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13737.56 Da        Isoelectric Point: 7.0268

>AT241494 WP_001280950.1 NZ_CP095137:c4216784-4216416 [Escherichia coli]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAFYPTSETKK

Download         Length: 369 bp

>AT241494 NZ_LT992472:626633-626693 [Staphylococcus aureus]
TACATAATAAATTGAACATCTAAATACACCAAATCCCCTCACTACTGCCATAGTGAGGGGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1J6XFW9

Antitoxin

Source ID Structure
AlphaFold DB A0A1M1N885

References