Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4145094..4145670 Replicon chromosome
Accession NZ_CP095137
Organism Escherichia coli strain Iso00225

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag MU414_RS20270 Protein ID WP_001297643.1
Coordinates 4145383..4145670 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag MU414_RS20265 Protein ID WP_000063156.1
Coordinates 4145094..4145396 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MU414_RS20250 (4141690) 4141690..4143840 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
MU414_RS20255 (4143890) 4143890..4144093 + 204 WP_000467859.1 YbdD/YjiX family protein -
MU414_RS20260 (4144104) 4144104..4145060 + 957 WP_001297640.1 GTPase -
MU414_RS20265 (4145094) 4145094..4145396 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
MU414_RS20270 (4145383) 4145383..4145670 - 288 WP_001297643.1 BrnT family toxin Toxin
MU414_RS20275 (4145869) 4145869..4146566 - 698 Protein_3974 IS1 family transposase -
MU414_RS20280 (4146623) 4146623..4146718 + 96 Protein_3975 dienelactone hydrolase family protein -
MU414_RS20285 (4146864) 4146864..4148096 + 1233 WP_001339512.1 multidrug efflux MFS transporter MdtM -
MU414_RS20290 (4148137) 4148137..4149417 + 1281 WP_001037382.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T241493 WP_001297643.1 NZ_CP095137:c4145670-4145383 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T241493 NZ_LT992472:c331087-330983 [Staphylococcus aureus]
TTGTTACTCCTAGAAAGGACTAGCATGTCTGATTTTGAAATGCTGATGGTTGTATTAACAATCATTGGTTTAGTATTGAT
TAGTACTCAAGACCATAAAAAATAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT241493 WP_000063156.1 NZ_CP095137:c4145396-4145094 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT241493 NZ_LT992472:330889-330927 [Staphylococcus aureus]
AACATGTTACCCTAATGAGCCCGTTAAAAAGACGGTGAC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References