Detailed information of TA system TA241476

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 869400..870234
• Replicon: chromosome
• Accession: NZ_CP095137
• Organism: Escherichia coli strain Iso00225

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: MU414_RS04240
• Protein ID: WP_001546109.1
• Coordinates: 869400..869777 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A7I0L0C8
• Locus tag: MU414_RS04245
• Protein ID: WP_001546108.1
• Coordinates: 869854..870234 (-)
• Length: 127 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MU414_RS04210 (865803)	865803..865973	-	171	Protein_829	IS110 family transposase	-
MU414_RS04215 (866390)	866390..867324	-	935	Protein_830	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
MU414_RS04220 (867317)	867317..867712	-	396	WP_000208383.1	DUF6088 family protein	-
MU414_RS04225 (867781)	867781..868625	-	845	Protein_832	DUF4942 domain-containing protein	-
MU414_RS04230 (868710)	868710..868898	-	189	WP_001545730.1	DUF957 domain-containing protein	-
MU414_RS04235 (868915)	868915..869403	-	489	WP_000761698.1	DUF5983 family protein	-
MU414_RS04240 (869400)	869400..869777	-	378	WP_001546109.1	TA system toxin CbtA family protein	Toxin
MU414_RS04245 (869854)	869854..870234	-	381	WP_001546108.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
MU414_RS04250 (870284)	870284..870928	-	645	WP_000086755.1	hypothetical protein	-
MU414_RS04255 (870947)	870947..871168	-	222	WP_000692345.1	DUF987 domain-containing protein	-
MU414_RS04260 (871237)	871237..871713	-	477	WP_042962214.1	RadC family protein	-
MU414_RS04265 (871729)	871729..872214	-	486	WP_000849588.1	antirestriction protein	-
MU414_RS04270 (872269)	872269..873087	-	819	WP_001234620.1	DUF932 domain-containing protein	-
MU414_RS04275 (873187)	873187..873420	-	234	WP_001119729.1	DUF905 family protein	-
MU414_RS04280 (873499)	873499..873954	-	456	WP_001545736.1	IrmA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13986.00 Da
• Isoelectric Point: 8.5190

>T241476 WP_001546109.1 NZ_CP095137:c869777-869400 [Escherichia coli]
MKTLPDTHVRAASRCPSPVTIWQTLLTQLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRNNYKMVNNITQGKHPEAKR

Nucleotide

• Length: 378 bp

>T241476 NZ_LT992470:2485263-2485472 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATTGATGGTCAAGTAACGGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATTTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin

Protein

• Length: 127 a.a.
• Molecular weight: 13997.78 Da
• Isoelectric Point: 5.0823

>AT241476 WP_001546108.1 NZ_CP095137:c870234-869854 [Escherichia coli]
MSDTLPGTTLPDDNKDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFNNADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCDADTLGSCGYVYLAVYPATETESNPPE

Nucleotide

• Length: 381 bp

>AT241476 NZ_LT992470:2484958-2485251 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGATAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGAGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Structures

Antitoxin

• AlphaFold DB: A0A7I0L0C8