Detailed information of TA system TA241470

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 9765..10501
• Replicon: plasmid pIso00267_02
• Accession: NZ_CP095134
• Organism: Klebsiella pneumoniae strain Iso00267

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: L7SZ15
• Locus tag: MU415_RS27630
• Protein ID: WP_003026803.1
• Coordinates: 10019..10501 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: MU415_RS27625
• Protein ID: WP_003026799.1
• Coordinates: 9765..10031 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MU415_RS27570	4913..5236	-	324	WP_022644730.1	hypothetical protein	-
MU415_RS27575	5263..5658	-	396	WP_017899885.1	hypothetical protein	-
MU415_RS27580	5827..6132	-	306	WP_211656811.1	type II toxin-antitoxin system toxin CcdB	-
MU415_RS27585	6134..6352	-	219	WP_211656810.1	type II toxin-antitoxin system antitoxin CcdA	-
MU415_RS27590	6947..7216	+	270	WP_004152102.1	hypothetical protein	-
MU415_RS27595	7204..7779	+	576	WP_004152103.1	hypothetical protein	-
MU415_RS27600	7810..8304	+	495	WP_004152104.1	DNA-binding protein	-
MU415_RS27605	8348..8716	+	369	WP_004152105.1	hypothetical protein	-
MU415_RS27610	8750..8953	+	204	WP_004152106.1	HHA domain-containing protein	-
MU415_RS27615	9002..9259	+	258	WP_004152107.1	hypothetical protein	-
MU415_RS27620	9335..9589	+	255	WP_004152108.1	hypothetical protein	-
MU415_RS27625	9765..10031	+	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
MU415_RS27630	10019..10501	+	483	WP_003026803.1	GNAT family N-acetyltransferase	Toxin
MU415_RS27635	10660..10857	-	198	Protein_18	IS3 family transposase	-
MU415_RS27640	10971..11939	+	969	WP_077265821.1	IS5 family transposase	-
MU415_RS27645	11995..12342	+	348	Protein_20	IS6-like element IS26 family transposase	-
MU415_RS27650	12388..12810	-	423	Protein_21	alpha/beta hydrolase-fold protein	-
MU415_RS27655	12832..13941	-	1110	WP_000842134.1	S-(hydroxymethyl)glutathione dehydrogenase/class III alcohol dehydrogenase	-
MU415_RS27660	14036..15220	-	1185	WP_001039466.1	tetracycline efflux MFS transporter Tet(D)	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	tet(D) / catA2 / aph(6)-Id / aph(3'')-Ib / sul2 / aac(6')-Ib-cr / ARR-3 / dfrA27 / aadA16 / qacE / sul1	-	1..70936	70936

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17353.97 Da
• Isoelectric Point: 9.5822

>T241470 WP_003026803.1 NZ_CP095134:10019-10501 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T241470 NZ_LT992469:2607857-2607964 [Staphylococcus aureus]
ATGTTCAATTTATTAATTGACATCATGACTTCAGCTTTAAGCGGCTGTCTTGTTGCGTTTTTTGCACATTGGTTACGAAC
GCGCAACAATAAAAAAGGTGACAAATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT241470 WP_003026799.1 NZ_CP095134:9765-10031 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT241470 NZ_LT992469:c2608041-2607981 [Staphylococcus aureus]
TACATAATAAATTGAACATCTAAATACACCAAATCCCCTCACTACTGCCATAGTGAGGGGA

Structures

Toxin

• AlphaFold DB: A0A0J2GNW6

Antitoxin

• AlphaFold DB: A0A1Q8YL66