Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 57318..58054 Replicon plasmid pIso00267_01
Accession NZ_CP095133
Organism Klebsiella pneumoniae strain Iso00267

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag MU415_RS26805 Protein ID WP_003026803.1
Coordinates 57572..58054 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag MU415_RS26800 Protein ID WP_003026799.1
Coordinates 57318..57584 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MU415_RS26775 52460..52951 + 492 WP_032249453.1 hypothetical protein -
MU415_RS26780 53592..54560 - 969 WP_245146868.1 IS5-like element IS903B family transposase -
MU415_RS26785 55242..55550 - 309 WP_017896554.1 hypothetical protein -
MU415_RS26790 56044..56592 + 549 WP_001567366.1 thioredoxin fold domain-containing protein -
MU415_RS26795 56639..57073 + 435 WP_001567367.1 cell envelope integrity protein TolA -
MU415_RS26800 57318..57584 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
MU415_RS26805 57572..58054 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
MU415_RS26810 58266..59612 + 1347 WP_020314316.1 ISNCY family transposase -
MU415_RS28105 59773..59904 + 132 WP_004218042.1 hypothetical protein -
MU415_RS26815 60113..61278 - 1166 Protein_59 IS3 family transposase -
MU415_RS26820 61455..62417 - 963 WP_004152113.1 zinc metalloprotease HtpX -
MU415_RS26825 62404..62892 - 489 WP_004152114.1 phosphate-starvation-inducible PsiE family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aac(3)-IIa / blaTEM-1B / blaCTX-M-15 - 1..194916 194916
- inside IScluster/Tn - - 53592..61140 7548


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T241468 WP_003026803.1 NZ_CP095133:57572-58054 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T241468 NZ_LT992469:c1274379-1274272 [Staphylococcus aureus]
GTGATATCTATTGCAAATGCATTACATTTAATGTTAAGTTTCGGTATGTTTATCGTCACTTTCATTGGTGTAGTAGTCGC
AATAATTAATTTAAACAATAAAAAATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT241468 WP_003026799.1 NZ_CP095133:57318-57584 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT241468 NZ_LT992469:1274182-1274219 [Staphylococcus aureus]
AACATGTCGCCTAATGAGCCCGTTAAAAAGACGGTGAC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References