Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5370872..5371497 Replicon chromosome
Accession NZ_CP095132
Organism Klebsiella pneumoniae strain Iso00267

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag MU415_RS26160 Protein ID WP_002882817.1
Coordinates 5370872..5371255 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag MU415_RS26165 Protein ID WP_004150355.1
Coordinates 5371255..5371497 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MU415_RS26145 (5368238) 5368238..5369140 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
MU415_RS26150 (5369137) 5369137..5369772 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
MU415_RS26155 (5369769) 5369769..5370698 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
MU415_RS26160 (5370872) 5370872..5371255 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
MU415_RS26165 (5371255) 5371255..5371497 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
MU415_RS26170 (5371702) 5371702..5372619 + 918 WP_004178029.1 alpha/beta hydrolase -
MU415_RS26175 (5372633) 5372633..5373574 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
MU415_RS26180 (5373619) 5373619..5374056 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
MU415_RS26185 (5374053) 5374053..5374913 - 861 WP_004146232.1 virulence factor BrkB family protein -
MU415_RS26190 (5374907) 5374907..5375506 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T241467 WP_002882817.1 NZ_CP095132:c5371255-5370872 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T241467 NZ_LT992469:c712208-711747 [Staphylococcus aureus]
ATGGGATTATATGAAGAAACTTTAATACAACATGATTATATTGAAATAAGAGAGGCTGATGTGCTTCCAGATAATTTGGA
TGGGGTATGGTTAGGAGATTTAATTTTAATAAAGCGTGGCTTATCAGATAGAGAAAAGGCAGGAATTCTCTTTGAAGAAT
TAGCACATAATAAACTTACATACGGTGATATAGCCGATTACTCGAAATTCAACAATCGCAAGTTCGAAAATTACGCAAGG
CGACACGGCTTTATCTCAGCAGTCCCGTTACGCGAAATTGTGGAAGCTTACAATTATGGTGTACGTAACTTGTATGAGTT
GTCTGAGTATCTGCAATTGAGTGAAGAATACATATTAGAAGCAATAGAACAATATAAAAAGATATATGGTATTGGGACTC
ACTACGGCGAATACTCAATTACATTTGAGCCATTGAGAGTTTTTAAATATAAAGAAATATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT241467 WP_004150355.1 NZ_CP095132:c5371497-5371255 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT241467 NZ_LT992469:c712535-712221 [Staphylococcus aureus]
ATGTGCTTTTCAAAAAGAATGAAACAATCAAGAGAAAAACAAGGTATGACTTTGGCCGAACTAGGAAGAAAAATTGGTAA
AACTGAAGCTACTGTACAGCGTTATGAAAGCGGAAATATCAAAAATTTAAAAAACGATACTATAGAAAGTATAGCTACTG
CATTAAATGTTAATCCTGCATATTTAATGGGGTGGGTTGAAGAAAACGATGATGAAGTACAACATCGTGCAGCTCACCTT
GAAGGAGAATTGACAGATGATGAATGGCAAAGAGTTTTAGATTATGCAGATTATATAAGAAGCAAACGTAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References