Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicB(antitoxin)
Location 3312109..3312740 Replicon chromosome
Accession NZ_CP095132
Organism Klebsiella pneumoniae strain Iso00267

Toxin (Protein)


Gene name hicA Uniprot ID A0A1Q8YTV3
Locus tag MU415_RS16320 Protein ID WP_012542177.1
Coordinates 3312564..3312740 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A3Q9U6R4
Locus tag MU415_RS16315 Protein ID WP_017898984.1
Coordinates 3312109..3312516 (-) Length 136 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MU415_RS16275 (3307554) 3307554..3307976 - 423 WP_077256680.1 hypothetical protein -
MU415_RS16280 (3307973) 3307973..3308290 - 318 WP_064162882.1 hypothetical protein -
MU415_RS16285 (3308242) 3308242..3308591 - 350 Protein_3206 HNH endonuclease signature motif containing protein -
MU415_RS16290 (3308745) 3308745..3308969 - 225 WP_226938768.1 DNA gyrase subunit B -
MU415_RS16295 (3309074) 3309074..3309262 - 189 WP_071888095.1 hypothetical protein -
MU415_RS16300 (3310338) 3310338..3310688 - 351 WP_201524646.1 hypothetical protein -
MU415_RS16305 (3310685) 3310685..3311182 - 498 WP_017898985.1 lysozyme -
MU415_RS16310 (3311182) 3311182..3311397 - 216 WP_017880269.1 class II holin family protein -
MU415_RS16315 (3312109) 3312109..3312516 - 408 WP_017898984.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
MU415_RS16320 (3312564) 3312564..3312740 - 177 WP_012542177.1 type II toxin-antitoxin system HicA family toxin Toxin
MU415_RS16325 (3313105) 3313105..3314889 + 1785 WP_032415155.1 ATP-binding protein -
MU415_RS16330 (3314909) 3314909..3315943 + 1035 WP_071600377.1 DNA cytosine methyltransferase -
MU415_RS16335 (3315968) 3315968..3316309 - 342 WP_017898982.1 antiterminator Q family protein -
MU415_RS16340 (3316322) 3316322..3317353 - 1032 WP_017898981.1 DUF968 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 3281863..3328035 46172


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-61)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6632.83 Da        Isoelectric Point: 11.0174

>T241462 WP_012542177.1 NZ_CP095132:c3312740-3312564 [Klebsiella pneumoniae]
VKQSELRRWLAAQGAEFKDGTNHLKIYLNGKQTVMPRHPGKEIPEPLRKAILKQLGIK

Download         Length: 177 bp

>T241462 NZ_LT992469:75195-75299 [Staphylococcus aureus]
TTGTTACTCCTAGAAAGGACTAGCATGTCTGATTTTGAAATGCTGATGGTTGTATTAACAATCATTGGTTTAGTATTGAT
TAGTACTCAAGACCATAAAAAATAA

Antitoxin


Download         Length: 136 a.a.        Molecular weight: 14959.96 Da        Isoelectric Point: 4.4277

>AT241462 WP_017898984.1 NZ_CP095132:c3312516-3312109 [Klebsiella pneumoniae]
MRYPVIFEHDETGWAVFFPDIPEAMTGGETREEALEMAQDALVTAFDFYFDDRREIPAPSAEGDAFVEVPASVAAKVLLL
NRLVSTNTSNADLARLINTRPQEVQRIVSLGHSTKIDTIQKALSALGQKMEIVVH

Download         Length: 408 bp

>AT241462 NZ_LT992469:c75458-75294 [Staphylococcus aureus]
GTAGTAAGTAGAAGCAAAAGATGAAAATCTTTAACTCTTGAAACACAAAAAGGGCAACACTCGGAAACATGTTACCCTAA
TGAGCCCGTTAAAAAGACGGTGACCTTATATTTTATTTAAAAATAGCCTTCAAAAATGCCGGTCAAAGCGAATAGAAGGT
TATTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q8YTV3

Antitoxin

Source ID Structure
AlphaFold DB A0A3Q9U6R4

References