Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 53945..54588 Replicon plasmid pIso00270_03
Accession NZ_CP095128
Organism Escherichia coli strain Iso00270

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag MU416_RS23920 Protein ID WP_001044768.1
Coordinates 54172..54588 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag MU416_RS23915 Protein ID WP_001261287.1
Coordinates 53945..54175 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MU416_RS23905 (49123) 49123..50256 - 1134 WP_000545983.1 DUF3800 domain-containing protein -
MU416_RS23910 (50519) 50519..53638 - 3120 WP_023909028.1 hypothetical protein -
MU416_RS23915 (53945) 53945..54175 + 231 WP_001261287.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
MU416_RS23920 (54172) 54172..54588 + 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
MU416_RS23925 (54750) 54750..56888 - 2139 WP_000350635.1 AAA family ATPase -
MU416_RS23930 (57353) 57353..58348 + 996 WP_000246636.1 hypothetical protein -
MU416_RS23935 (58391) 58391..59284 + 894 WP_001553853.1 S-4TM family putative pore-forming effector -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid tet(B) / catA1 / blaCTX-M-15 - 1..64118 64118


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T241448 WP_001044768.1 NZ_CP095128:54172-54588 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

>T241448 NZ_LT992467:c767137-767033 [Staphylococcus aureus]
TTGTTACTCCTAGAAAGGACTAGCATGTCTGATTTTGAAATGCTGATGGTTGTATTAACAATCATTGGTTTAGTATTGAT
TAGTACTCAAGACCATAAAAAATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT241448 WP_001261287.1 NZ_CP095128:53945-54175 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT241448 NZ_LT992467:766939-766977 [Staphylococcus aureus]
AACATGTTACCCTAATGAGCCCGTTAAAAAGACGGTGAC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References