Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 38843..39444 Replicon plasmid pIso00270_02
Accession NZ_CP095127
Organism Escherichia coli strain Iso00270

Toxin (Protein)


Gene name doc Uniprot ID A0A5M4JUN5
Locus tag MU416_RS23205 Protein ID WP_001216041.1
Coordinates 38843..39223 (-) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag MU416_RS23210 Protein ID WP_001190712.1
Coordinates 39223..39444 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MU416_RS23175 34865..35893 + 1029 WP_245112242.1 tyrosine-type recombinase/integrase -
MU416_RS23180 36003..36311 + 309 WP_000349257.1 hypothetical protein -
MU416_RS23185 36308..36784 + 477 WP_245112245.1 hypothetical protein -
MU416_RS23190 36781..37275 + 495 WP_000640903.1 dUTP diphosphatase -
MU416_RS23195 37290..37952 + 663 WP_000255077.1 DUF2829 domain-containing protein -
MU416_RS23200 37959..38759 + 801 WP_000432092.1 hypothetical protein -
MU416_RS23205 38843..39223 - 381 WP_001216041.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
MU416_RS23210 39223..39444 - 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
MU416_RS23215 39517..39906 - 390 WP_000506731.1 S24 family peptidase -
MU416_RS23220 40005..40256 - 252 WP_032220968.1 DNA polymerase III subunit theta -
MU416_RS23225 40289..40639 + 351 WP_245112247.1 hypothetical protein -
MU416_RS23230 40679..40894 - 216 WP_024259737.1 hypothetical protein -
MU416_RS23235 40991..41821 - 831 WP_118940355.1 DUF551 domain-containing protein -
MU416_RS23240 41818..42027 - 210 WP_005025300.1 hypothetical protein -
MU416_RS23245 42029..42217 - 189 WP_000797282.1 hypothetical protein -
MU416_RS23250 42390..42836 - 447 WP_088551336.1 ead/Ea22-like family protein -
MU416_RS23255 42833..43528 - 696 WP_106104759.1 ead/Ea22-like family protein -
MU416_RS23260 43525..44169 - 645 WP_245112252.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..94223 94223


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(3-120)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13635.52 Da        Isoelectric Point: 5.6406

>T241445 WP_001216041.1 NZ_CP095127:c39223-38843 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGIQVYDSPVLVELAVGAATGEIPVSSVAEKLRELFGSNI

Download         Length: 381 bp

>T241445 NZ_LT992467:c592298-592122 [Staphylococcus aureus]
TTGGATAGATGGTGGCTATCTGAGTATAAGGAGGTGGTGCCTATGTTGGCATTACTGAAATCTTTAGAAAGGAGACGCCT
AATGATTACAATTAGTACCATGTTGCAGTTTGGTTTATTCCTTATTGCATTGATAGGTCTAGTAATCAAGCTTATTGAAT
TAAGCAATAAAAAATAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT241445 WP_001190712.1 NZ_CP095127:c39444-39223 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT241445 NZ_LT992467:591991-592132 [Staphylococcus aureus]
ATATATAGAAAAAGGGCAACATGCGCAAACATGTTACCCTAGTGAGCCCGTTAAAAAGACGGTGGCTATTTTAGATTAAA
GATTAAATTAATAACCATTTAACCATCGAAACCAGCCAAAGTTAGCGATGGTTATTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5M4JUN5

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References