Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 68529..69130 Replicon plasmid pIso00270_01
Accession NZ_CP095126
Organism Escherichia coli strain Iso00270

Toxin (Protein)


Gene name doc Uniprot ID U9YA20
Locus tag MU416_RS22750 Protein ID WP_001216045.1
Coordinates 68529..68909 (-) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag MU416_RS22755 Protein ID WP_001190712.1
Coordinates 68909..69130 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MU416_RS22725 63969..65453 - 1485 WP_000124155.1 hypothetical protein -
MU416_RS22730 65453..66646 - 1194 WP_000219604.1 terminase -
MU416_RS22735 66733..67107 - 375 WP_024166509.1 hypothetical protein -
MU416_RS22740 67274..68317 - 1044 WP_000648825.1 DUF968 domain-containing protein -
MU416_RS22745 68345..68524 - 180 WP_023153731.1 hypothetical protein -
MU416_RS22750 68529..68909 - 381 WP_001216045.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
MU416_RS22755 68909..69130 - 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
MU416_RS22760 69203..69592 - 390 WP_000506726.1 S24 family peptidase -
MU416_RS22765 69716..69967 - 252 WP_001377386.1 DNA polymerase III subunit theta -
MU416_RS22770 70417..70554 + 138 WP_000123562.1 hypothetical protein -
MU416_RS22775 70629..70991 - 363 WP_001261544.1 hypothetical protein -
MU416_RS22780 70988..71920 - 933 WP_000057449.1 hypothetical protein -
MU416_RS22785 71902..72276 - 375 WP_000988658.1 hypothetical protein -
MU416_RS22790 72283..72576 - 294 WP_001677496.1 hypothetical protein -
MU416_RS22795 72755..72988 - 234 WP_000516537.1 hypothetical protein -
MU416_RS24160 73071..73367 - 297 WP_224690240.1 DUF551 domain-containing protein -
MU416_RS22805 73635..73814 - 180 Protein_76 hypothetical protein -
MU416_RS22810 73830..73958 - 129 Protein_77 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..99933 99933


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(3-120)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13588.29 Da        Isoelectric Point: 5.1514

>T241444 WP_001216045.1 NZ_CP095126:c68909-68529 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T241444 NZ_LT992467:c477595-477443 [Staphylococcus aureus]
ATGTCTAAAGACAAAGATCCAAAATTAAATTATCATGAAGAAGAAAACAGTATGGTAACTGATTTTGAAGATTTAAAAGA
ATTAGGTAAAGAAATGGAACAAATTTCTGATCAAAATGATCAAGAAAAGAATTCTGAAGAAGACAGTCAGTAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT241444 WP_001190712.1 NZ_CP095126:c69130-68909 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT241444 NZ_LT992467:c478219-477620 [Staphylococcus aureus]
ATGGCAATGAACTTTAAAGTCTTTGACAATAGTCAACTTGTAGCAGAATATGCTGCTGATATTATTAGAAAGCAATTTAA
CAATAATCCTACTACAATTGCAGGTTTTCATTTAGATACAGATCAAGCGCCAGTACTAGATGAATTAAAGAAAAATGTAG
AAAAACATGCTGTTGATTTTAGCCAAATAAATATTTTAGATTATGACGATAAAAAATCATATTTCGAAGCGTTAGGTGTA
CCAGCAGGTCAAGTTTATCCAATTGCTTATGAAAAAGATGCAATCGAATTAATCGCTGATAAGATTAAAACTAAAGAAAA
TAAAGGGAAATTAACATTACAAGTTGTTTCTATCGATGAACAAGGTAAGTTAAATGTTAGTATTCGTCAAGGACTAATGG
AAGCAAGAGAAATTTTCTTAGTAGTGACTGGTGCTAATAAACGAGATGTAGTTGAAAAATTATATCAAGAAAATGGTAAA
ACAAGCTTTGAACCAGCCGATTTAAAAGCACATAGAATGGTAAATGTTATTCTTGATAAAGAAGCGGCTGCAGGTTTACC
CGAAGATGTTAAAGCTTACTTTACGTCACGCTTTGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLP7

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References