Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 3992223..3992799 Replicon chromosome
Accession NZ_CP095125
Organism Escherichia coli strain Iso00270

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag MU416_RS19215 Protein ID WP_001297643.1
Coordinates 3992512..3992799 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag MU416_RS19210 Protein ID WP_000063156.1
Coordinates 3992223..3992525 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MU416_RS19195 (3988819) 3988819..3990969 + 2151 WP_001446922.1 pyruvate/proton symporter BtsT -
MU416_RS19200 (3991019) 3991019..3991222 + 204 WP_000467859.1 YbdD/YjiX family protein -
MU416_RS19205 (3991233) 3991233..3992189 + 957 WP_001297640.1 GTPase -
MU416_RS19210 (3992223) 3992223..3992525 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
MU416_RS19215 (3992512) 3992512..3992799 - 288 WP_001297643.1 BrnT family toxin Toxin
MU416_RS19220 (3992998) 3992998..3993695 - 698 Protein_3770 IS1 family transposase -
MU416_RS19225 (3993752) 3993752..3993847 + 96 Protein_3771 dienelactone hydrolase family protein -
MU416_RS19230 (3993993) 3993993..3995225 + 1233 WP_001380906.1 multidrug efflux MFS transporter MdtM -
MU416_RS19235 (3995266) 3995266..3996546 + 1281 WP_001037380.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 3992998..3993207 209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T241442 WP_001297643.1 NZ_CP095125:c3992799-3992512 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T241442 NZ_LT992466:c2038847-2038740 [Staphylococcus aureus]
GTGATATCTATTGCAAATGCATTACATTTAATGTTAAGTTTCGGTATGTTTATCGTCACTTTCATTGGTGTAGTAGTCGC
AATAATTAATTTAAACAATAAAAAATAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT241442 WP_000063156.1 NZ_CP095125:c3992525-3992223 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT241442 NZ_LT992466:2038650-2038687 [Staphylococcus aureus]
AACATGTCGCCTAATGAGCCCGTTAAAAAGACGGTGAC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References