Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 861646..862300 Replicon chromosome
Accession NZ_CP095125
Organism Escherichia coli strain Iso00270

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag MU416_RS04215 Protein ID WP_000244777.1
Coordinates 861893..862300 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag MU416_RS04210 Protein ID WP_000354046.1
Coordinates 861646..861912 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MU416_RS04185 (856815) 856815..857558 + 744 WP_000951964.1 SDR family oxidoreductase -
MU416_RS04190 (857615) 857615..859048 - 1434 WP_001446971.1 6-phospho-beta-glucosidase BglA -
MU416_RS04195 (859093) 859093..859404 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
MU416_RS04200 (859568) 859568..860227 + 660 WP_000250274.1 hemolysin III family protein -
MU416_RS04205 (860423) 860423..861403 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
MU416_RS04210 (861646) 861646..861912 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
MU416_RS04215 (861893) 861893..862300 + 408 WP_000244777.1 protein YgfX Toxin
MU416_RS04220 (862340) 862340..862861 - 522 WP_001055874.1 flavodoxin FldB -
MU416_RS04225 (862973) 862973..863869 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
MU416_RS04230 (863894) 863894..864604 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
MU416_RS04235 (864610) 864610..866343 + 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T241426 WP_000244777.1 NZ_CP095125:861893-862300 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T241426 NZ_LT992464:2314935-2315396 [Staphylococcus aureus]
ATGGGATTATATGAAGAAACTTTAATACAACATGATTATATTGAAATAAGAGAGGCTGATGTGCTTCCAGATAATTTGGA
TGGGGTATGGTTAGGAGATTTAATTTTAATAAAGCGTGGCTTATCAGATAGAGAAAAGGCAGGAATTCTCTTTGAAGAAT
TAGCACATAATAAACTTACATACGGTGATATAGCCGATTACTCGAAATTCAACAATCGCAAGTTCGAAAATTACGCAAGG
CGACACGGCTTTATCTCAGCAGTCCCGTTACGCGAAATTGTGGAAGCTTACAATTATGGTGTACGTAACTTGTATGAGTT
GTCTGAGTATCTGCAATTGAGTGAAGAATACATATTAGAAGCAATAGAACAATATAAAAAGATATATGGTATTGGGACTC
ACTACGGCGAATACTCAATTACATTTGAGCCATTGAGAGTTTTTAAATATAAAGAAATATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT241426 WP_000354046.1 NZ_CP095125:861646-861912 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT241426 NZ_LT992464:2314608-2314922 [Staphylococcus aureus]
ATGTGCTTTTCAAAAAGAATGAAACAATCAAGAGAAAAACAAGGTATGACTTTGGCCGAACTAGGAAGAAAAATTGGTAA
AACTGAAGCTACTGTACAGCGTTATGAAAGCGGAAATATCAAAAATTTAAAAAACGATACTATAGAAAGTATAGCTACTG
CATTAAATGTTAATCCTGCATATTTAATGGGGTGGGTTGAAGAAAACGATGATGAAGTACAACATCGTGCAGCTCACCTT
GAAGGAGAATTGACAGATGATGAATGGCAAAGAGTTTTAGATTATGCAGATTATATAAGAAGCAAACGTAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References