Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5158691..5159316 Replicon chromosome
Accession NZ_CP095121
Organism Klebsiella pneumoniae strain KP1601

Toxin (Protein)


Gene name vapC Uniprot ID A0A378FVD4
Locus tag MUU71_RS24960 Protein ID WP_019705794.1
Coordinates 5158691..5159074 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag MUU71_RS24965 Protein ID WP_004150355.1
Coordinates 5159074..5159316 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MUU71_RS24945 (5156057) 5156057..5156959 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
MUU71_RS24950 (5156956) 5156956..5157591 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
MUU71_RS24955 (5157588) 5157588..5158517 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
MUU71_RS24960 (5158691) 5158691..5159074 - 384 WP_019705794.1 type II toxin-antitoxin system VapC family toxin Toxin
MUU71_RS24965 (5159074) 5159074..5159316 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
MUU71_RS24970 (5159521) 5159521..5160438 + 918 WP_004146235.1 alpha/beta hydrolase -
MUU71_RS24975 (5160453) 5160453..5161394 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
MUU71_RS24980 (5161439) 5161439..5161876 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
MUU71_RS24985 (5161873) 5161873..5162733 - 861 WP_004146232.1 virulence factor BrkB family protein -
MUU71_RS24990 (5162727) 5162727..5163326 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14358.57 Da        Isoelectric Point: 6.8806

>T241421 WP_019705794.1 NZ_CP095121:c5159074-5158691 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGHREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T241421 NZ_LT992464:581499-581675 [Staphylococcus aureus]
TTGGATAGATGGTGGCTATCTGAGTATAAGGAGATGGTGCCTATGGTGGCATTACTGAAATCTTTAGAAAGGAGACGCCT
AATGATTACAATTAGTACCATGTTGCAGTTTGGGTTATTCCTTATTGCATTGATAGGTCTAGTAATCAAGCTTATTGAAT
TAAGCAATAAAAAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT241421 WP_004150355.1 NZ_CP095121:c5159316-5159074 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT241421 NZ_LT992464:c581837-581663 [Staphylococcus aureus]
ATTTGATATTTATATTATGGTGTGTTAATTTATATATAGAAAAAGGGCAACATGCGCAAACATGTTACCCTAATGAGCCC
GTTAAAAAGACGGTGGCTATTTTAGATTAAAGATTAAATTAATAACCATTTAACCATCGAAACCAGCCAAAGTTAGCGAT
GGTTATTTTTTATTG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A378FVD4

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References