Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 449136..449754 Replicon chromosome
Accession NC_012971
Organism Escherichia coli BL21(DE3)

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag ECD_RS02150 Protein ID WP_001291435.1
Coordinates 449136..449354 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag ECD_RS02155 Protein ID WP_000344800.1
Coordinates 449380..449754 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECD_RS02120 444425..444997 + 573 WP_000779826.1 lipoprotein -
ECD_RS02125 445028..445339 - 312 WP_000409911.1 MGMT family protein -
ECD_RS02130 445718..446071 + 354 WP_000878140.1 DUF1428 domain-containing protein -
ECD_RS02135 446113..447663 - 1551 WP_001319841.1 cyclic-guanylate-specific phosphodiesterase PdeB -
ECD_RS02140 447827..448297 - 471 WP_000136192.1 YlaC family protein -
ECD_RS02145 448413..448964 - 552 WP_000102564.1 maltose O-acetyltransferase -
ECD_RS02150 449136..449354 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
ECD_RS02155 449380..449754 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
ECD_RS02160 450300..453449 - 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
ECD_RS02165 453472..454665 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T24142 WP_001291435.1 NC_012971:c449354-449136 [Escherichia coli BL21(DE3)]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T24142 NC_012971:c449354-449136 [Escherichia coli BL21(DE3)]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT24142 WP_000344800.1 NC_012971:c449754-449380 [Escherichia coli BL21(DE3)]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT24142 NC_012971:c449754-449380 [Escherichia coli BL21(DE3)]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References