Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4507300..4508110 Replicon chromosome
Accession NZ_CP095121
Organism Klebsiella pneumoniae strain KP1601

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag MUU71_RS21880 Protein ID WP_004178461.1
Coordinates 4507300..4507833 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag MUU71_RS21885 Protein ID WP_002887278.1
Coordinates 4507844..4508110 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MUU71_RS21875 (4506131) 4506131..4507252 + 1122 WP_004186850.1 cupin domain-containing protein -
MUU71_RS21880 (4507300) 4507300..4507833 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
MUU71_RS21885 (4507844) 4507844..4508110 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
MUU71_RS21890 (4508213) 4508213..4509646 - 1434 WP_004186848.1 Cu(+)/Ag(+) sensor histidine kinase -
MUU71_RS21895 (4509636) 4509636..4510319 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
MUU71_RS21900 (4510492) 4510492..4511877 + 1386 WP_064152126.1 efflux transporter outer membrane subunit -
MUU71_RS21905 (4511895) 4511895..4512239 + 345 WP_064152125.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T241418 WP_004178461.1 NZ_CP095121:c4507833-4507300 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T241418 NZ_LT992464:409936-410040 [Staphylococcus aureus]
TTGTTACTCCTAGAAAGGACTAGCATGTCTGATTTTGAAATGCTGATGGTTGTATTAACAATCATTGGTTTAGTATTGAT
TAGTACTCAAGACCATAAAAAATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT241418 WP_002887278.1 NZ_CP095121:c4508110-4507844 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT241418 NZ_LT992464:c410199-410035 [Staphylococcus aureus]
GTAGTAAGTAGAAGCAAAAGATGAAAATCTTTAACTCTTGAAACACAAAAAGGGCAACACTCGGAAACATGTTACCCTAA
TGAGCCCGTTAAAAAGACGGTGACCTTATATTTTATTTAAAAATAGCCTTCAAAAATGCCGGTCAAAGCGAATAGAAGGT
TATTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References