Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 413475..414312 Replicon chromosome
Accession NC_012971
Organism Escherichia coli BL21(DE3)

Toxin (Protein)


Gene name itaT Uniprot ID A0A140NB16
Locus tag ECD_RS01975 Protein ID WP_000227786.1
Coordinates 413475..414017 (-) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag ECD_RS01980 Protein ID WP_001297137.1
Coordinates 414001..414312 (-) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECD_RS01955 409005..409916 - 912 WP_000705853.1 2-dehydropantoate 2-reductase -
ECD_RS01960 410084..410575 + 492 WP_001138904.1 nucleotide binding protein YajQ -
ECD_RS01965 410703..412067 - 1365 WP_001000978.1 MFS transporter -
ECD_RS01970 412484..413419 + 936 WP_001297127.1 sel1 repeat family protein -
ECD_RS01975 413475..414017 - 543 WP_000227786.1 GNAT family N-acetyltransferase Toxin
ECD_RS01980 414001..414312 - 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
ECD_RS01985 414497..415387 - 891 WP_000971336.1 heme o synthase -
ECD_RS01990 415399..415728 - 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
ECD_RS01995 415728..416342 - 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
ECD_RS02000 416332..418323 - 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
ECD_RS02005 418345..419292 - 948 WP_001239440.1 cytochrome o ubiquinol oxidase subunit II -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19794.98 Da        Isoelectric Point: 8.3395

>T24141 WP_000227786.1 NC_012971:c414017-413475 [Escherichia coli BL21(DE3)]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADSAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

>T24141 NC_012971:c414017-413475 [Escherichia coli BL21(DE3)]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACTCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT24141 WP_001297137.1 NC_012971:c414312-414001 [Escherichia coli BL21(DE3)]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp

>AT24141 NC_012971:c414312-414001 [Escherichia coli BL21(DE3)]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A140NB16

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References