Detailed information of TA system TA241398

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2198405..2198934
• Replicon: chromosome
• Accession: NZ_CP095119
• Organism: Staphylococcus aureus strain IVB6154

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: MUA68_RS11250
• Protein ID: WP_000621175.1
• Coordinates: 2198405..2198767 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: MUA68_RS11255
• Protein ID: WP_000948331.1
• Coordinates: 2198764..2198934 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MUA68_RS11230 (2195383)	2195383..2196153	-	771	WP_001041107.1	RNA polymerase sigma factor SigB	-
MUA68_RS11235 (2196128)	2196128..2196607	-	480	WP_001190825.1	anti-sigma B factor RsbW	-
MUA68_RS11240 (2196609)	2196609..2196935	-	327	WP_001052491.1	anti-sigma factor antagonist	-
MUA68_RS11245 (2197054)	2197054..2198055	-	1002	WP_178890286.1	PP2C family protein-serine/threonine phosphatase	-
MUA68_RS11250 (2198405)	2198405..2198767	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
MUA68_RS11255 (2198764)	2198764..2198934	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
MUA68_RS11260 (2199019)	2199019..2200167	-	1149	WP_262520400.1	alanine racemase	-
MUA68_RS11265 (2200233)	2200233..2200592	-	360	WP_000581197.1	holo-ACP synthase	-
MUA68_RS11270 (2200596)	2200596..2201087	-	492	WP_078316666.1	PH domain-containing protein	-
MUA68_RS11275 (2201074)	2201074..2202661	-	1588	Protein_2186	PH domain-containing protein	-
MUA68_RS11280 (2202654)	2202654..2203133	-	480	WP_262520401.1	hypothetical protein	-
MUA68_RS11285 (2203342)	2203342..2203902	-	561	WP_262520402.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T241398 WP_000621175.1 NZ_CP095119:c2198767-2198405 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T241398 NZ_LT992462:c752014-751763 [Staphylococcus aureus]
ATGAATAATCGCGAACAAATTGAACAATCAGTTATAAGTGCTAGTGCGTATAACGGTAATGACACAGAGGGATTACTAAA
AGAGATTGAGGACGTTTATAAGAAAGCGCAAGCGTTTGATGAAATACTTGAGGGAATGACAAATGCTATTCAACATTCAG
TTAAAGAAGGTGTTGAACTTGATGAAGCAGTAGGGATTATGGCAGGTCAAGTTGTCTATAAATATGAGGAGGAACAGGAA
AATGAGCATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT241398 WP_000948331.1 NZ_CP095119:c2198934-2198764 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT241398 NZ_LT992462:c751773-751591 [Staphylococcus aureus]
ATGAGCATTAGTGTAGGAGACAAGGTTTTTAATCCAGAAACAAATTCAACTTTAGAAATTGTACAACTTGTTGGCGATAT
TAGAGACACGCATTACAAGTTATCTGACGGATCTATTATTAGTCTTATAGACTTTGTTGTTAAACCAATTCATTTAATCA
AGGAGGAGCAGGAAAATGACTAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1