Detailed information of TA system TA024136

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 4513377..4514191
• Replicon: chromosome
• Accession: NC_012967
• Organism: Escherichia coli B str. REL606

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: S1PA82
• Locus tag: ECB_RS22215
• Protein ID: WP_001054376.1
• Coordinates: 4513934..4514191 (-)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: U9Z4B8
• Locus tag: ECB_RS22210
• Protein ID: WP_001309181.1
• Coordinates: 4513377..4513922 (-)
• Length: 182 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECB_RS22190	4509068..4510381	-	1314	WP_000460843.1	galactitol-specific PTS transporter subunit IIC	-
ECB_RS22195	4510393..4510671	-	279	WP_000722973.1	PTS sugar transporter subunit IIB	-
ECB_RS22200	4510668..4511789	-	1122	WP_000010829.1	M42 family metallopeptidase	-
ECB_RS25820	4512034..4512150	-	117	Protein_4218	VOC family protein	-
ECB_RS25275	4512188..4512406	-	219	Protein_4219	hypothetical protein	-
ECB_RS22205	4512575..4513321	-	747	WP_000354251.1	class I SAM-dependent methyltransferase	-
ECB_RS22210	4513377..4513922	-	546	WP_001309181.1	N-acetyltransferase	Antitoxin
ECB_RS22215	4513934..4514191	-	258	WP_001054376.1	hypothetical protein	Toxin
ECB_RS25675	4514568..4514813	-	246	Protein_4223	GNAT family N-acetyltransferase	-
ECB_RS22230	4514929..4516169	+	1241	Protein_4224	helicase YjhR	-
ECB_RS22235	4516752..4517732	-	981	WP_000991438.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
ECB_RS22240	4517797..4518903	-	1107	WP_001309184.1	N-acetylneuraminate epimerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (18-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9734.29 Da
• Isoelectric Point: 11.0090

>T24136 WP_001054376.1 NC_012967:c4514191-4513934 [Escherichia coli B str. REL606]
MNLSRQEQHTLHVLAKGRRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTELNKVRA
QLDNR

Nucleotide

• Length: 258 bp

>T24136 NC_012967:c4514191-4513934 [Escherichia coli B str. REL606]
ATGAATTTATCCCGTCAGGAACAACATACCTTACACGTTCTCGCTAAAGGTAGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin

Protein

• Length: 182 a.a.
• Molecular weight: 19956.90 Da
• Isoelectric Point: 6.3277

>AT24136 WP_001309181.1 NC_012967:c4513922-4513377 [Escherichia coli B str. REL606]
MTVHHFTFHITDKSDASDIREVETRAFGFSKEADLVASLLEDESARPALSLLARYEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQSLTA
QPMTLTGHIRCADPDETGALT

Nucleotide

• Length: 546 bp

>AT24136 NC_012967:c4513922-4513377 [Escherichia coli B str. REL606]
ATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCAGCAAAGAAGCCGATTTAGTGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCCGCGTTATCGCTGCTAGCCC
GGTATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCAACCTTCAAAGGAGAAATGGATTCACCGTTGATGCAC
ATCCTCGCTCCCCTGGCGGTCATACCGGAATACCAAGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAATCACTCACCGCT
CAGCCCATGACCCTGACCGGCCACATCCGGTGCGCAGACCCTGATGAAACCGGAGCACTGACGTGA

Structures

Toxin

• AlphaFold DB: S1PA82

Antitoxin

• AlphaFold DB: U9Z4B8