Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4331304..4331801 Replicon chromosome
Accession NC_012967
Organism Escherichia coli B str. REL606

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag ECB_RS21285 Protein ID WP_001173343.1
Coordinates 4331628..4331801 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag ECB_RS21280 Protein ID WP_000398619.1
Coordinates 4331304..4331600 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECB_RS21260 4328030..4328749 - 720 WP_000611288.1 two-component system response regulator DcuR -
ECB_RS21265 4328746..4330382 - 1637 Protein_4041 sensor histidine kinase -
ECB_RS21270 4330563..4330793 + 231 WP_001070602.1 (4Fe-4S)-binding protein -
ECB_RS21275 4330805..4331077 + 273 WP_000405651.1 N-acetyltransferase -
ECB_RS21280 4331304..4331600 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
ECB_RS21285 4331628..4331801 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
ECB_RS21290 4331920..4333437 - 1518 WP_001295090.1 lysine--tRNA ligase -
ECB_RS21295 4333674..4335131 - 1458 WP_000856841.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T24135 WP_001173343.1 NC_012967:4331628-4331801 [Escherichia coli B str. REL606]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T24135 NC_012967:4331628-4331801 [Escherichia coli B str. REL606]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT24135 WP_000398619.1 NC_012967:4331304-4331600 [Escherichia coli B str. REL606]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT24135 NC_012967:4331304-4331600 [Escherichia coli B str. REL606]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References