Detailed information of TA system TA241322

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsdAT/-
• Location: 1814009..1814523
• Replicon: chromosome
• Accession: NZ_CP095104
• Organism: Staphylococcus simulans strain IVB6179

Toxin (Protein)

• Gene name: TsdT
• Uniprot ID: A0A133QQ97
• Locus tag: MUA12_RS09255
• Protein ID: WP_002481764.1
• Coordinates: 1814009..1814215 (-)
• Length: 69 a.a.

Antitoxin (Protein)

• Gene name: TsdA
• Uniprot ID: -
• Locus tag: MUA12_RS09260
• Protein ID: WP_262577160.1
• Coordinates: 1814230..1814523 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MUA12_RS09235 (MUA12_09190)	1810009..1811217	+	1209	WP_262577156.1	FtsW/RodA/SpoVE family cell cycle protein	-
MUA12_RS09240 (MUA12_09195)	1811277..1811417	-	141	WP_023016106.1	Lmo0850 family protein	-
MUA12_RS09245 (MUA12_09200)	1811499..1813691	-	2193	WP_262577158.1	heavy metal translocating P-type ATPase	-
MUA12_RS09250 (MUA12_09205)	1813784..1813990	-	207	WP_002481763.1	copper ion binding protein	-
MUA12_RS09255 (MUA12_09210)	1814009..1814215	-	207	WP_002481764.1	heavy metal-associated domain-containing protein	Toxin
MUA12_RS09260 (MUA12_09215)	1814230..1814523	-	294	WP_262577160.1	copper-sensing transcriptional repressor CsoR	Antitoxin
MUA12_RS09265 (MUA12_09220)	1814682..1816163	+	1482	WP_023016108.1	cardiolipin synthase	-
MUA12_RS09270 (MUA12_09225)	1816187..1816837	+	651	WP_262577161.1	HD domain-containing protein	-
MUA12_RS09275 (MUA12_09230)	1816895..1817407	-	513	WP_070848611.1	GNAT family N-acetyltransferase	-
MUA12_RS09280 (MUA12_09235)	1817533..1818402	-	870	WP_262577162.1	membrane protein insertase YidC	-
MUA12_RS09285 (MUA12_09240)	1818491..1819129	-	639	WP_107547577.1	thiamine phosphate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Heavy metal-associated domain, HMA (24-65)

Antitoxin

• Metal-sensitive transcriptional repressor (14-91)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7861.82 Da
• Isoelectric Point: 4.2670

>T241322 WP_002481764.1 NZ_CP095104:c1814215-1814009 [Staphylococcus simulans]
MHSSVIQISGMETEEQQHILQEHLQQMIGMSEVQVDLKSQEVKASYQTPISLNNLEKEIYDQGYQILN

Nucleotide

• Length: 207 bp

>T241322 NZ_LT991957:900197-900496 [Enterobacter cloacae complex sp.]
ATGATTAATCATTTTCGTGATCAATGGCTTAAGGATTTTTTCCTGTATGGGAAATCCAGCAATGTTATCCCTTCAAGCCT
TGAATCAGCCTTAGCGAGGAAGCTGGATATGATCAACGCTGCCACCTCGCACCGGGATCTGAGATCCCCGCCAGGCAATA
TGTATGAATCGCTGAATCCCCCGCTTGCAGGGTACTCGGCGATTCGCGTGAACAGGCAATACAGGTTGGTATTCAGGTGG
TCAGAGGGCAAAGCAGACGATCTCTACCTCTCTCCGCACAAGTACACGCCATACAAATGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10975.66 Da
• Isoelectric Point: 7.3855

>AT241322 WP_262577160.1 NZ_CP095104:c1814523-1814230 [Staphylococcus simulans]
MTQEERAHHSEKIKSNLKARLNRIEGQVRAINRMIDEDVYCDDVLTQIRATRSALNSVAVKLLDHHMKGCILHKAEDGQA
EAAMAELLVTVQKLIKD

Nucleotide

• Length: 294 bp

>AT241322 NZ_LT991957:900507-900854 [Enterobacter cloacae complex sp.]
ATGACTCTTCAACAGGCTCTACGCAAACCCACCACGCCGGGCGACGTGTTGCAGTATGAATATCTTGAGCCGCTCAATCT
GAAAATTAGCGATCTTGCGGAAATGCTTAACGTGCACCGTAACACCATCAGTGCTCTCGTTAATAATAATCGTAAACTGA
CGGCCGATATGGCATTCAGACTGGCCAAGGCGTTTGATACCACCATTGAATTCTGGTTGAACCTACAGCTAAACGTCGAC
GTCTGGGAAGCGCAAACCAATTCCCGGACTCAGGAGGAGTTGAGCCGTATAAAGACGGTCGCGGAAATTATGGCAAAAAG
GAAATCAGGCCAGCCGGATGTGGCCTGA

Structures

Toxin

• AlphaFold DB: A0A133QQ97