Detailed information of TA system TA241319

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 986330..986844
• Replicon: chromosome
• Accession: NZ_CP095102
• Organism: Staphylococcus simulans strain IVB6188

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: MUA56_RS04390
• Protein ID: WP_031262808.1
• Coordinates: 986494..986844 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A2T4QNU3
• Locus tag: MUA56_RS04385
• Protein ID: WP_023016097.1
• Coordinates: 986330..986497 (+)
• Length: 56 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MUA56_RS04360 (MUA56_04365)	982220..982684	+	465	WP_023016102.1	PH domain-containing protein	-
MUA56_RS04365 (MUA56_04370)	982677..984170	+	1494	WP_107587564.1	PH domain-containing protein	-
MUA56_RS04370 (MUA56_04375)	984163..984660	+	498	WP_023016100.1	PH domain-containing protein	-
MUA56_RS04375 (MUA56_04380)	984662..985015	+	354	WP_031262810.1	holo-ACP synthase	-
MUA56_RS04380 (MUA56_04385)	985095..986249	+	1155	WP_262551330.1	alanine racemase	-
MUA56_RS04385 (MUA56_04390)	986330..986497	+	168	WP_023016097.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
MUA56_RS04390 (MUA56_04395)	986494..986844	+	351	WP_031262808.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
MUA56_RS04395 (MUA56_04400)	987136..988137	+	1002	WP_002481750.1	SpoIIE family protein phosphatase	-
MUA56_RS04400 (MUA56_04405)	988213..988539	+	327	WP_002481749.1	anti-sigma factor antagonist	-
MUA56_RS04405 (MUA56_04410)	988541..989020	+	480	WP_262551331.1	anti-sigma B factor RsbW	-
MUA56_RS04410 (MUA56_04415)	988995..989765	+	771	WP_002481747.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-109)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12976.09 Da
• Isoelectric Point: 10.0474

>T241319 WP_031262808.1 NZ_CP095102:986494-986844 [Staphylococcus simulans]
MMRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKSKYKLDKDSVILLEQIR
TVDKKRLKEKLTYLSDEKMKEIDNAIGISLGLTHKN

Nucleotide

• Length: 351 bp

>T241319 NZ_LT991956:105936-106223 [Enterobacter hormaechei subsp. steigerwaltii]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 56 a.a.
• Molecular weight: 6257.78 Da
• Isoelectric Point: 3.6836

>AT241319 WP_023016097.1 NZ_CP095102:986330-986497 [Staphylococcus simulans]
MSTFNQNRSYSLEQSLKEGYSQMADLNLSLATEAFSVECEACDCNESYLTFDKDE

Nucleotide

• Length: 168 bp

>AT241319 NZ_LT991956:105697-105936 [Enterobacter hormaechei subsp. steigerwaltii]
ATGGGTAGCATTAACCTACGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGCGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGACTTTGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Antitoxin

• AlphaFold DB: A0A2T4QNU3