Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2926169..2926823 Replicon chromosome
Accession NC_012967
Organism Escherichia coli B str. REL606

Toxin (Protein)


Gene name cptA Uniprot ID A0A140N853
Locus tag ECB_RS14430 Protein ID WP_000244783.1
Coordinates 2926169..2926576 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag ECB_RS14435 Protein ID WP_000354046.1
Coordinates 2926557..2926823 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECB_RS14410 2922126..2923859 - 1734 WP_000813212.1 single-stranded-DNA-specific exonuclease RecJ -
ECB_RS14415 2923865..2924575 - 711 WP_000715216.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
ECB_RS14420 2924600..2925496 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
ECB_RS14425 2925608..2926129 + 522 WP_001055874.1 flavodoxin FldB -
ECB_RS14430 2926169..2926576 - 408 WP_000244783.1 protein YgfX Toxin
ECB_RS14435 2926557..2926823 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
ECB_RS14440 2927066..2928046 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
ECB_RS14445 2928242..2928901 - 660 WP_000250274.1 hemolysin III family protein -
ECB_RS14450 2929065..2929376 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
ECB_RS14455 2929421..2930854 + 1434 WP_012775976.1 6-phospho-beta-glucosidase BglA -
ECB_RS14460 2930911..2931654 - 744 WP_000951948.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 15962.85 Da        Isoelectric Point: 11.2669

>T24131 WP_000244783.1 NC_012967:c2926576-2926169 [Escherichia coli B str. REL606]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEISLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T24131 NC_012967:c2926576-2926169 [Escherichia coli B str. REL606]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTAGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT24131 WP_000354046.1 NC_012967:c2926823-2926557 [Escherichia coli B str. REL606]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT24131 NC_012967:c2926823-2926557 [Escherichia coli B str. REL606]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A140N853

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References