Detailed information of TA system TA241307

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 1671482..1672080
• Replicon: chromosome
• Accession: NZ_CP095094
• Organism: Flavonifractor plautii strain VE303-08

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: K5I25_RS08405
• Protein ID: WP_055271538.1
• Coordinates: 1671482..1671820 (-)
• Length: 113 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A4R2LWB8
• Locus tag: K5I25_RS08410
• Protein ID: WP_021630507.1
• Coordinates: 1671814..1672080 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K5I25_RS08380 (K5I25_08380)	1666726..1666986	+	261	WP_024725199.1	DUF6103 family protein	-
K5I25_RS08385 (K5I25_08385)	1666973..1668430	+	1458	WP_195309244.1	ankyrin repeat domain-containing protein	-
K5I25_RS08390 (K5I25_08390)	1668427..1669242	+	816	WP_246961020.1	hypothetical protein	-
K5I25_RS08395 (K5I25_08395)	1669565..1669990	+	426	WP_178863548.1	hypothetical protein	-
K5I25_RS08400 (K5I25_08400)	1670005..1671435	+	1431	WP_246961023.1	MobP3 family relaxase	-
K5I25_RS08405 (K5I25_08405)	1671482..1671820	-	339	WP_055271538.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
K5I25_RS08410 (K5I25_08410)	1671814..1672080	-	267	WP_021630507.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
K5I25_RS08415 (K5I25_08415)	1672158..1673081	+	924	WP_055271541.1	DUF3991 and toprim domain-containing protein	-
K5I25_RS08420 (K5I25_08420)	1673211..1674962	+	1752	WP_227111847.1	type IV secretory system conjugative DNA transfer family protein	-
K5I25_RS08425 (K5I25_08425)	1674959..1675237	+	279	WP_009261352.1	helix-turn-helix domain-containing protein	-
K5I25_RS08430 (K5I25_08430)	1675435..1675917	-	483	WP_021631604.1	sigma-70 family RNA polymerase sigma factor	-
K5I25_RS08435 (K5I25_08435)	1676287..1677000	-	714	WP_009261353.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1650376..1790284	139908

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (6-101)

Antitoxin

• SpoVT-AbrB domain (6-41)

Sequences

Toxin

Protein

• Length: 113 a.a.
• Molecular weight: 12766.54 Da
• Isoelectric Point: 5.5275

>T241307 WP_055271538.1 NZ_CP095094:c1671820-1671482 [Flavonifractor plautii]
MVMIFEQGDIVYLDFDPQSGHEQKSRRPALVISNNLFNRVSSLTMVCPITHTDRGHPFHLRLDERTRTDGVILCDQARML
DLNSRHASFVERAPEDISAEAVDMVIGFLEPK

Nucleotide

• Length: 339 bp

>T241307 NZ_LT991954:c1941635-1941417 [Enterobacter hormaechei subsp. steigerwaltii]
ATGTCTGATAAACCATTAACAAAAGTCGATTATTTGATGCGCCTGCGACGCTGCCAGTCAATTGACACCCTTGAACGAGT
TATTGAGAAAAATAAATACGAGCTGTCTGATAATGAACTGGCGGTATTTTATTCAGCTGCCGACCATCGTCTTGCTGAGC
TGACCATGAATAAACTGTATGACAAGATCCCCTCTTCGGTGTGGAAATTTGTTCGTTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9960.28 Da
• Isoelectric Point: 5.1473

>AT241307 WP_021630507.1 NZ_CP095094:c1672080-1671814 [Flavonifractor plautii]
MYATIQKWGNSQGLRIPKALLDAVGLKEHDRVELVQMDDGINIRRASATPHRTLEERLIAFYGKPIDQIGRIEGEELDWG
KAEGSEAW

Nucleotide

• Length: 267 bp

>AT241307 NZ_LT991954:c1942037-1941663 [Enterobacter hormaechei subsp. steigerwaltii]
ATGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGAAGAAAGTAACCATGGCTGGGTGAACGATCCAACGTCTGCTATCAATTTACAGCTTAACGAGCTGATCGAGC
ATATCGCCACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGTTGATAGAGCAAATTGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTACGGCATTAACGCACAGGATTTGCAGAAATGGCGTAAATCAGGAAACCGTCT
ATTTCGCTGCTTCACTAATGTCAGCAGAGCTAATCCGGTTAGTCTTTCCTGTTAA

Structures

Antitoxin

• AlphaFold DB: A0A4R2LWB8