Detailed information of TA system TA241305

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 590823..591421
• Replicon: chromosome
• Accession: NZ_CP095094
• Organism: Flavonifractor plautii strain VE303-08

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G9YW30
• Locus tag: K5I25_RS02830
• Protein ID: WP_007494762.1
• Coordinates: 590823..591161 (-)
• Length: 113 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G9YW29
• Locus tag: K5I25_RS02835
• Protein ID: WP_007494759.1
• Coordinates: 591155..591421 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K5I25_RS02795 (K5I25_02795)	586014..586724	+	711	WP_009261531.1	hypothetical protein	-
K5I25_RS02800 (K5I25_02800)	586918..587067	+	150	Protein_534	hypothetical protein	-
K5I25_RS02805 (K5I25_02805)	587274..587762	+	489	WP_009261530.1	hypothetical protein	-
K5I25_RS02810 (K5I25_02810)	587759..588574	+	816	WP_024723145.1	hypothetical protein	-
K5I25_RS02815 (K5I25_02815)	588896..589318	+	423	WP_024723144.1	hypothetical protein	-
K5I25_RS02820 (K5I25_02820)	589315..589629	+	315	WP_021630164.1	hypothetical protein	-
K5I25_RS02825 (K5I25_02825)	589829..590776	+	948	WP_226923944.1	MobP3 family relaxase	-
K5I25_RS02830 (K5I25_02830)	590823..591161	-	339	WP_007494762.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
K5I25_RS02835 (K5I25_02835)	591155..591421	-	267	WP_007494759.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
K5I25_RS23420	591865..592185	+	321	Protein_542	DUF3991 and TOPRIM domain-containing protein	-
K5I25_RS23425	592152..592424	+	273	WP_242864858.1	toprim domain-containing protein	-
K5I25_RS02845 (K5I25_02845)	592554..594305	+	1752	Protein_544	type IV secretory system conjugative DNA transfer family protein	-
K5I25_RS02850 (K5I25_02850)	594302..594505	+	204	WP_007494404.1	helix-turn-helix domain-containing protein	-
K5I25_RS02855 (K5I25_02855)	594650..595927	-	1278	WP_007494406.1	ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-96)

Antitoxin

• Antitoxin PemI-like (1-88)

Sequences

Toxin

Protein

• Length: 113 a.a.
• Molecular weight: 12722.43 Da
• Isoelectric Point: 5.5275

>T241305 WP_007494762.1 NZ_CP095094:c591161-590823 [Flavonifractor plautii]
MVTIFEQGDIVYLDFDPQSGHEQKGRRPALVISNNLFNRVSSLTMVCPITHTDRGHPFHLRLDERTRTDGVILCDQARML
DLNSRHASFVERAPEDISSEAVDMVIGFLEPK

Nucleotide

• Length: 339 bp

>T241305 NZ_LT991954:c646692-646507 [Enterobacter hormaechei subsp. steigerwaltii]
GTGAAAAGTGCGGATATCATTGCTGTTCTGGTTAGCCATGGCTGGAAATGTGTAAGAACGACGGGAAGCCATCATCAGTT
TCGCCATCCAGTGCAAAAAGGGTTGGTAACCGTTCCGCATCCTAAAAAGGACATTAAACCGGGCACTCTCGCACAGATCT
GGCGACAGGCGGGCATTAAACACTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10048.34 Da
• Isoelectric Point: 4.8668

>AT241305 WP_007494759.1 NZ_CP095094:c591421-591155 [Flavonifractor plautii]
MYATIQKWGNSQGLRIPKALLDAVGLKEHDRVELVQMDDGINIRRASSIPHRTLEERLTAFYGKPIDQIDRIEAEELDWG
KAEGSEAW

Nucleotide

• Length: 267 bp

>AT241305 NZ_LT991954:c646492-646085 [Enterobacter hormaechei subsp. steigerwaltii]
ATGTTTTATCCGGCTTACATTCATTCTGATTCCGACGGTAGCGCCAGCGGTTTCTTCCCCGACGTTCCGGGATGTTTTTT
TGCGGGGGATTCACTTGATGACGCTTTTCAGGATGCACGTGATGCGCTCACGGCGCACTTCGAAGCGCTGTTTGAAATGG
ATGAAGCATTTCCTCTCCCGGGTAATGTCGAAGTGCACCTTGAAAATCAACCTGACGACTTCACAGGCGGGCAATGGTTG
CTGGTGGATATTAATATGAAGCAATTCGACGGCAGAGCCGAACGCATCAACATCACGATGCCTCGACGTTTGCTGGTGAA
GATTGATTCCTTTGTCAGCGAGCATCCTCAATTCGGCAATCGAAGCGCATTTCTGGCCGAGGCAGCACGTCGCGTGCTGC
CTCGGTGA

Structures

Toxin

• AlphaFold DB: A0A174IQD2

Antitoxin

• AlphaFold DB: A0A174ME76