241297

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-DnaT
Location 10020..10542 Replicon plasmid pW264
Accession NZ_CP095089
Organism Escherichia coli isolate w264

Toxin (Protein)


Gene name relE Uniprot ID D0QMR1
Locus tag MUK78_RS23015 Protein ID WP_000220561.1
Coordinates 10261..10542 (+) Length 94 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID G3CAG1
Locus tag MUK78_RS23010 Protein ID WP_000121743.1
Coordinates 10020..10271 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MUK78_RS22990 (MUK78_22990) 6143..6496 - 354 WP_024129958.1 DNA distortion polypeptide 3 -
MUK78_RS22995 (MUK78_22995) 6633..7079 - 447 WP_001074384.1 hypothetical protein -
MUK78_RS23000 (MUK78_23000) 7083..7925 - 843 WP_244756217.1 replication initiation protein -
MUK78_RS23005 (MUK78_23005) 8093..8785 - 693 WP_244756218.1 replication initiation protein -
MUK78_RS23010 (MUK78_23010) 10020..10271 + 252 WP_000121743.1 plasmid stabilization protein Antitoxin
MUK78_RS23015 (MUK78_23015) 10261..10542 + 282 WP_000220561.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
MUK78_RS23020 (MUK78_23020) 10781..10873 - 93 Protein_15 IS6 family transposase -
MUK78_RS23025 (MUK78_23025) 11039..12238 - 1200 WP_000948429.1 IS91 family transposase -
MUK78_RS23030 (MUK78_23030) 12248..12436 - 189 WP_000957857.1 hypothetical protein -
MUK78_RS23035 (MUK78_23035) 12556..13986 + 1431 Protein_18 IS4-like element IS50R family transposase -
MUK78_RS23040 (MUK78_23040) 14015..14809 + 795 WP_000572405.1 aminoglycoside O-phosphotransferase APH(3')-IIa -
MUK78_RS23045 (MUK78_23045) 14830..15066 + 237 Protein_20 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aph(3')-IIa / sul2 / aph(3'')-Ib / aph(6)-Id / mph(A) / aac(3)-IId - 1..36460 36460


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 11023.86 Da        Isoelectric Point: 10.5938

>T241297 WP_000220561.1 NZ_CP095089:10261-10542 [Escherichia coli]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADMR

Download         Length: 282 bp

>T241297 NZ_LT985192:c157028-156744 [Pseudomonas syringae]
ATGAAAGAACAAATTTTCGATTACGACCCAGCAGCAGCCCTTGACGGTCCTGAAGCAATCGCGATTTTTATCTCTGATGC
CTTTGAGACGGGGGATGCGGAGTACATCGCGAAGGCTATGGGGGTTGTCGCAAGGGCCAAAGGGATGACCGAGCTTGCGC
GCGAGACAGGTCTGTCTCGAGAGCAGCTTTATCGGTCGTTCAGTAGCAAGGGCAATCCAACCTTAAAAACCATGTTGACG
GTAATGAGAGCCCTGGGCGTGGATATGACAGCTCGAATGCACTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9194.43 Da        Isoelectric Point: 4.1683

>AT241297 WP_000121743.1 NZ_CP095089:10020-10271 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Download         Length: 252 bp

>AT241297 NZ_LT985192:c157324-157025 [Pseudomonas syringae]
ATGACGACGATAAAACAGACCTCTACCTATATGGCTTGGGAACGCAGGCTTAAAGACCAGAAGGCCAAAGCTGCAATTGC
CGCTCGTATTTTTAGAGTTGCAAATGGCCTGATGGGTGATGTCTCTCCAGTAGGACAGGGCGTTAGTGAGCTACGGATAC
ACGTTGGCCCTGGTTACCGTGTTTATTTCCAGCAGCGAGGCGATGAACTGATCTTGCTGCTGTGCGGTGGTGACAAGAGC
AGCCAATCCCGAGATATCGAAACAGCCAAGAAACTGGCTGATCAATGGTGGCAAGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3S5I302

Antitoxin

Source ID Structure
AlphaFold DB A0A3S5I301

References