Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4042400..4042976 Replicon chromosome
Accession NZ_CP095088
Organism Escherichia coli isolate w264

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag MUK78_RS19800 Protein ID WP_001297643.1
Coordinates 4042689..4042976 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A080I7H7
Locus tag MUK78_RS19795 Protein ID WP_000063160.1
Coordinates 4042400..4042702 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MUK78_RS19780 (4038996) 4038996..4041146 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
MUK78_RS19785 (4041196) 4041196..4041399 + 204 WP_000467859.1 YbdD/YjiX family protein -
MUK78_RS19790 (4041410) 4041410..4042366 + 957 WP_001297640.1 GTPase -
MUK78_RS19795 (4042400) 4042400..4042702 - 303 WP_000063160.1 BrnA antitoxin family protein Antitoxin
MUK78_RS19800 (4042689) 4042689..4042976 - 288 WP_001297643.1 BrnT family toxin Toxin
MUK78_RS23270 (4043175) 4043175..4043871 - 697 Protein_3885 IS1 family transposase -
MUK78_RS19815 (4044186) 4044186..4045418 + 1233 WP_001137030.1 multidrug efflux MFS transporter MdtM -
MUK78_RS19820 (4045459) 4045459..4046739 + 1281 WP_001037390.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4043175..4043384 209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T241294 WP_001297643.1 NZ_CP095088:c4042976-4042689 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T241294 NZ_LT985188:c2773106-2772789 [Micropruina glycogenica]
GTGCGCGAGATCTGTCTGGCCCGGTTGGACAAGACCCGTCCAGTGGTCGTACTGACCCGTGAGGCTGCCCGTGCCGCCAT
GACGAAGGTCACCGTCGCCCCGATCACCTCGACCATCAAGGGTCTCAATAGCGAAGTCTCGGTCGGGCCGGACAATGGTC
TCGAACAGCTGAGCGTGATCTCCCTTGACAACGTTGTGACCATCCCCGCCAACCTGCTCGGACGAACCGTCGGCTTCCTG
AGCTCCGAACAGGAAATGCTGCTGGCTAAGGCCATCGTCCTCGCCTACGACCTCGACATCGCCCTCCTCGACACCTGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11552.01 Da        Isoelectric Point: 9.4646

>AT241294 WP_000063160.1 NZ_CP095088:c4042702-4042400 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALTNKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT241294 NZ_LT985188:c2773341-2773108 [Micropruina glycogenica]
ATGAGCACCCAGATTGCAGTCCGGCTACCTGACGAGATGGTGGCTTTCCTCGACCAAGCCGTGGCCCAAGGCAAGGCCGC
CAGCCGCGCACAGCTGGTCAGTGCCGCGCTGGAGCGTGAGATGCGACGCCAGGCCGCCGAGGCCGACGCTGTGATCCTTC
GCCAGCAGGGTGCCGCCGATGATCTGGACCCACTGGTGGAGTGGACGGTCGGCCACCTCGACATCGCCGGCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A080I7H7

References