Detailed information of TA system TA241291

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 3573980..3574817
• Replicon: chromosome
• Accession: NZ_CP095088
• Organism: Escherichia coli isolate w264

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: MUK78_RS17595
• Protein ID: WP_000227784.1
• Coordinates: 3574275..3574817 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: MUK78_RS17590
• Protein ID: WP_001297137.1
• Coordinates: 3573980..3574291 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MUK78_RS17565 (3569000)	3569000..3569947	+	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-
MUK78_RS17570 (3569969)	3569969..3571960	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
MUK78_RS17575 (3571950)	3571950..3572564	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
MUK78_RS17580 (3572564)	3572564..3572893	+	330	WP_113396804.1	cytochrome o ubiquinol oxidase subunit IV	-
MUK78_RS17585 (3572905)	3572905..3573795	+	891	WP_000971336.1	heme o synthase	-
MUK78_RS17590 (3573980)	3573980..3574291	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
MUK78_RS17595 (3574275)	3574275..3574817	+	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
MUK78_RS17600 (3574873)	3574873..3575808	-	936	WP_001297127.1	tetratricopeptide repeat protein	-
MUK78_RS17605 (3576216)	3576216..3577580	+	1365	WP_001000978.1	MFS transporter	-
MUK78_RS17610 (3577708)	3577708..3578199	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
MUK78_RS17615 (3578367)	3578367..3579278	+	912	WP_000705874.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T241291 WP_000227784.1 NZ_CP095088:3574275-3574817 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T241291 NZ_LT985188:962771-963031 [Micropruina glycogenica]
GTGAACCTCGCCTTCACCCCGACCGGCTGGGACGACCTGAACTACTGGGCCGACAGGGATCGGGCGACCTACAAGAAGGT
CCGCGTGCTGCTGAAGGAAGTCGTGCGGACGCCGTTCGAGGGCACCGGGAAACCCGAGCCGCTGAAGTACCAGCTCGGCA
ACGTCTGGTCGCGTCGGATCACCCAGGAGCATCGACTGGTCTACGCGGTGGAGGACGAGCAGATCGTCGTGCTGGCGGCG
CGCTTCCACTACGGCGAGTGA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT241291 WP_001297137.1 NZ_CP095088:3573980-3574291 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT241291 NZ_LT985188:962508-962774 [Micropruina glycogenica]
ATGTCGACGATGAGTGCGAGCGAGGCGCGCAAGAACCTGTTCCCGTTGCTGGGACAGGTGAACGAGGACCACGACGTGGT
CCGGATCACATCCAAGGCCGGCAATGCTGTCCTGATCTCCGAGGCCGACTACGAGGCCTGGCAGACCACGCGGCATCTGT
TCTCCACTCCGGCGAATGCCCGCCGCCTGCTGGGCTCGATCCAGGAATGGCACGACGGCAACCTGGTCACCGAGGCGCTG
CCTGAGGACGCAGAAGGCGACGAGTGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3