Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 330014..330814 Replicon chromosome
Accession NZ_CP095088
Organism Escherichia coli isolate w264

Toxin (Protein)


Gene name ataT Uniprot ID F4NNI0
Locus tag MUK78_RS01545 Protein ID WP_000342449.1
Coordinates 330287..330814 (+) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag MUK78_RS01540 Protein ID WP_001277108.1
Coordinates 330014..330280 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MUK78_RS01520 (325672) 325672..326340 + 669 WP_000617723.1 cell division ATP-binding protein FtsE -
MUK78_RS01525 (326333) 326333..327391 + 1059 WP_001042003.1 permease-like cell division protein FtsX -
MUK78_RS01530 (327636) 327636..328490 + 855 WP_000130221.1 RNA polymerase sigma factor RpoH -
MUK78_RS01535 (328761) 328761..329864 + 1104 WP_001350438.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
MUK78_RS01540 (330014) 330014..330280 + 267 WP_001277108.1 type II toxin-antitoxin system antitoxin AtaR Antitoxin
MUK78_RS01545 (330287) 330287..330814 + 528 WP_000342449.1 type II toxin-antitoxin system toxin acetyltransferase AtaT Toxin
MUK78_RS01550 (330811) 330811..331194 - 384 WP_000778781.1 aspartate 1-decarboxylase autocleavage activator PanM -
MUK78_RS01555 (331618) 331618..332727 + 1110 WP_000827696.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
MUK78_RS01560 (332775) 332775..333701 + 927 WP_000003004.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
MUK78_RS01565 (333698) 333698..334975 + 1278 WP_000803799.1 branched chain amino acid ABC transporter permease LivM -
MUK78_RS01570 (334972) 334972..335739 + 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19691.70 Da        Isoelectric Point: 7.7457

>T241271 WP_000342449.1 NZ_CP095088:330287-330814 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

>T241271 NZ_LT963436:949569-949754 [Staphylococcus saprophyticus]
ATGAGTTTAGATAAAAATCAAAATAATCTACATCGTGATGAATTAGAGTCAGAAAACGCGATGGTTGATGATTTAGAGGA
TGTCGTAGAATTAGGAAAAGAAATGGAACAAATCTCTGAAGAAAATGATGAAGATAAATTAGATAAATCACATGACCCAG
CAGTACGTTCCGATTTAAATGAATAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT241271 WP_001277108.1 NZ_CP095088:330014-330280 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp

>AT241271 NZ_LT963436:948948-949547 [Staphylococcus saprophyticus]
ATGGCAATGAACTTTAAAGTTTTAGAAAATGAACAAGTTGTAGCGGAATATGCTGCAGATATCTTAAGAAAACAATTTAA
CAACAATCCAACAACAATTGCTGGCGTACACCTTTCAAGTGACAATGCACCAGTATTGGATGAATTAAAGAAAAATGTTG
ATAAACATGCAGTAGATTTTAGTCAAATTAATATTCTGGATTATGATAACAATAAATCATACTTTGAAGCACTTGGTGTT
CCAGAAGGCCAAATCTTCAATGTTTCATTTGGTGAAGATACGGAATCATTTATTAAAGATAAAATCAAAACTAAAGAAAA
TAAAGGGAAATTGATTACACAAGTACTTTCTATTGATACAAATGGTAAATTAGATGTAAGTGTGAATCAAGGTTTATTCT
CGGCACGTGAAGTTATTTTAATTATTACAGGTAGCGATAAAAAAGAAGTCGTTCATAAACTTTATGAAGAAAATGGTAAA
ACAAGTTTTGAACCTTCAGATTTAAAAGCGCACCGTATGGTTAATGTTATTTTAGATGAAGCGGCTGCTGAAGGTTTACC
AGAAGATGTTAGACATTATTTCACTGCACGTTTTGCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLZ4

Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References