Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 302164..302795 Replicon chromosome
Accession NZ_CP095088
Organism Escherichia coli isolate w264

Toxin (Protein)


Gene name hicA Uniprot ID U9Y2A7
Locus tag MUK78_RS01380 Protein ID WP_001259385.1
Coordinates 302164..302439 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID Q31VE4
Locus tag MUK78_RS01385 Protein ID WP_000593554.1
Coordinates 302436..302795 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MUK78_RS01365 (297168) 297168..298235 + 1068 WP_000361467.1 HlyD family secretion protein -
MUK78_RS01370 (298232) 298232..300967 + 2736 WP_000149165.1 ribosome-associated ATPase/putative transporter RbbA -
MUK78_RS01375 (300967) 300967..302091 + 1125 WP_001314210.1 ABC transporter permease -
MUK78_RS01380 (302164) 302164..302439 + 276 WP_001259385.1 type II toxin-antitoxin system HicA family toxin Toxin
MUK78_RS01385 (302436) 302436..302795 + 360 WP_000593554.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
MUK78_RS01390 (302845) 302845..303705 - 861 WP_001131783.1 class II aldolase -
MUK78_RS01395 (303737) 303737..304006 - 270 WP_000933060.1 HPr family phosphocarrier protein -
MUK78_RS01400 (303996) 303996..305504 - 1509 WP_001298718.1 FGGY-family carbohydrate kinase -
MUK78_RS01405 (305497) 305497..306855 - 1359 WP_001298729.1 PTS galactitol transporter subunit IIC -
MUK78_RS01410 (306932) 306932..307213 - 282 WP_000084022.1 PTS sugar transporter subunit IIB -
MUK78_RS01415 (307210) 307210..307683 - 474 WP_001161553.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 294547..309831 15284
- flank IS/Tn - - 307720..308097 377


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10199.94 Da        Isoelectric Point: 10.7076

>T241270 WP_001259385.1 NZ_CP095088:302164-302439 [Escherichia coli]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T241270 NZ_LT963436:819179-819541 [Staphylococcus saprophyticus]
ATGATGAGACGTGGTGACGTTTATTTAGCTGATTTATCACCAGTCCAGGGGTCTGAACAAGGGGGAATAAGACCTGTCGT
AATTATACAAAACGATACTGGTAATAAATATAGTCCTACCGTAATTGTAGCCGCAATTACTGGTAGGATAAATAAAGCTA
AAATACCTACACATGTAGAAATAGAAAAAAGTAAATATAAATTAGATAAAGATTCTGTTATATTGCTAGAGCAGATTAGA
ACTTTAGATAAAAATAGGCTGAAAGAAAAATTAACTTATCTTTCTGATAAAAAAATGAAAGAAGTTAATAATGCCATTGA
TATTAGTTTAGGATTACATACTATTAGGAGTCACAAATCCTGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13359.25 Da        Isoelectric Point: 4.8838

>AT241270 WP_000593554.1 NZ_CP095088:302436-302795 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLLEYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT241270 NZ_LT963436:819012-819182 [Staphylococcus saprophyticus]
ATGGCAAGTTTCAACCAAAGTAGAATTCATAGTATAGAACAACTTTTAAAAGAAGGCTATTCTCAAATGGCTGATTTGAA
CCTCTCCCTCGCAGCAGAAGCATTTCCGTTTGAATGTGAAGCTTGTGATTGCAACGAAGTATATATTAGCTCCAAGAATA
ATAATGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTW8

Antitoxin

Source ID Structure
AlphaFold DB A0A6H2GM94

References