Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4684685..4685182 Replicon chromosome
Accession NZ_CP095086
Organism Escherichia coli isolate EC5654

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag MUK77_RS22520 Protein ID WP_001173343.1
Coordinates 4684685..4684858 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID S1NQ59
Locus tag MUK77_RS22525 Protein ID WP_001312331.1
Coordinates 4684886..4685182 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MUK77_RS22510 (4681355) 4681355..4682812 + 1458 WP_000856828.1 dipeptide/tripeptide permease DtpC -
MUK77_RS22515 (4683049) 4683049..4684566 + 1518 WP_000003805.1 lysine--tRNA ligase -
MUK77_RS22520 (4684685) 4684685..4684858 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
MUK77_RS22525 (4684886) 4684886..4685182 - 297 WP_001312331.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
MUK77_RS22530 (4685411) 4685411..4685683 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
MUK77_RS22535 (4685695) 4685695..4685925 - 231 WP_000373291.1 4Fe-4S mono-cluster protein YjdI -
MUK77_RS22540 (4686106) 4686106..4687737 + 1632 WP_001216466.1 sensor histidine kinase -
MUK77_RS22545 (4687734) 4687734..4688453 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T241263 WP_001173343.1 NZ_CP095086:c4684858-4684685 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T241263 NZ_LT963410:70507-70782 [Pseudomonas syringae]
ATGGAATTGAAGTGGACAAGCAAGGCGCTTTCCGACATTACCAGGCTATACGAGTTTCTGGCCTTGGCGAATCAACCTGC
CGCAGCACGAGTAGTGCAGCAACTCACCACAGCACCTACCACGCTTCTGACTAACCCGCGCATTGGCGAACGCCTAGAGG
AGTTCGAGCCAAAGGACGTACGCCGAATTCAGATCGGCCAATACGAGATGCGTTACGAGATAGCGGATTCAACTATTTAC
CTGCTGCGCCTGTGGCACACCCGCGAAGACCGATAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11440.95 Da        Isoelectric Point: 4.1705

>AT241263 WP_001312331.1 NZ_CP095086:c4685182-4684886 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLSIMTVDDYFRQFYK

Download         Length: 297 bp

>AT241263 NZ_LT963410:70235-70507 [Pseudomonas syringae]
ATGGCAACCACCGCCAAAACCCGATCTGTCACAGCTCACGTTCCGGTTCAGCTGGCGGAAAAAGTCGATTTGATGGCTGA
GCGTCTGGAGCGCTCTAAGAACTGGATCGTCAAGCAAGCCCTGTCTGCTTGGATAGACCAGGAAGAAGAGCGTAGTCGCC
TGACCCGTGAGGCCTTGGCTGATGTTGACGCTGGACGCGTTATTGATCACCAAGCCGTCCAGGCCTGGGCTGACAGTCTT
AGCACCGACACTCCGTTGCCGGTGCCGCGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB S1NQ59

References