Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 4670996..4671797 Replicon chromosome
Accession NZ_CP095086
Organism Escherichia coli isolate EC5654

Toxin (Protein)


Gene name yeeV Uniprot ID D8EBK0
Locus tag MUK77_RS22470 Protein ID WP_001094436.1
Coordinates 4671420..4671797 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID B7MN76
Locus tag MUK77_RS22465 Protein ID WP_015953067.1
Coordinates 4670996..4671373 (+) Length 126 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MUK77_RS22430 (4666908) 4666908..4667588 + 681 WP_001282927.1 WYL domain-containing protein -
MUK77_RS22435 (4667736) 4667736..4668413 + 678 WP_001097312.1 hypothetical protein -
MUK77_RS22440 (4668419) 4668419..4668652 + 234 WP_001278287.1 DUF905 family protein -
MUK77_RS22445 (4668742) 4668742..4669560 + 819 WP_001175142.1 DUF932 domain-containing protein -
MUK77_RS22450 (4669651) 4669651..4670136 + 486 WP_000860054.1 antirestriction protein -
MUK77_RS22455 (4670151) 4670151..4670627 + 477 WP_001186756.1 RadC family protein -
MUK77_RS22460 (4670696) 4670696..4670917 + 222 WP_000692312.1 DUF987 domain-containing protein -
MUK77_RS22465 (4670996) 4670996..4671373 + 378 WP_015953067.1 type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA Antitoxin
MUK77_RS22470 (4671420) 4671420..4671797 + 378 WP_001094436.1 TA system toxin CbtA family protein Toxin
MUK77_RS22475 (4671794) 4671794..4672282 + 489 WP_000761714.1 DUF5983 family protein -
MUK77_RS22480 (4672294) 4672294..4672491 + 198 WP_000839254.1 DUF957 domain-containing protein -
MUK77_RS22485 (4672576) 4672576..4673421 + 846 WP_001280513.1 DUF4942 domain-containing protein -
MUK77_RS22490 (4673492) 4673492..4675027 + 1536 WP_000492897.1 EAL domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 13958.84 Da        Isoelectric Point: 6.8603

>T241262 WP_001094436.1 NZ_CP095086:4671420-4671797 [Escherichia coli]
MNTLPDTHVREASGCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRNNYRMVNNITQGKHPEAKQ

Download         Length: 378 bp

>T241262 NZ_LT963409:c5870455-5870105 [Pseudomonas syringae]
ATGAATACGTTTGCACTGCGCTTTACCGATCTTGCCCAGCAAAGCCTTGAAGACCAGGTTGAACACTTGGCTGTCACTCA
GGGATTCTCATCAGCTGCTCAACGCATAGATTCCCTTATCGATGCCATCCAGGACAAGCTCCTGTCCACCCCGTTGGGCT
ACCCGGTCAGTCCACAGTTGAGTGAGCTGGGTGTACTGCACTATCGTGAGTTGAACACTGACGGATACCGGATCTTTTAT
GAGGTGATGGATTCTGATGGTATTACCGATATCGCCGTATTGCTCGTATTAGGCGGGAAACAAAGTGTTGAACAAGCCCT
CATCCGCTATTGCCTTCTTCAGCCTATATAG

Antitoxin


Download         Length: 126 a.a.        Molecular weight: 13766.60 Da        Isoelectric Point: 6.2021

>AT241262 WP_015953067.1 NZ_CP095086:4670996-4671373 [Escherichia coli]
VSDTLPGTTHPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTITLYAKGLTCEADTLGSCGYVYLAVYPTPAAPAITV

Download         Length: 378 bp

>AT241262 NZ_LT963409:c5870716-5870468 [Pseudomonas syringae]
ATGCGAGTTGAAACAATAAGCTACCTGAAACGCAATGCAGCCGACCTGCCTCTCGATGAGCCATTGATCGTCACGCAAAA
CGGTGTCCCGGCCTACGTGGTGGAGTCTTACGCGGATCGTAAGCGCCGCGATGAGGCCATTGCCTTGGTAAAGCTCTTGG
CCATCAGTTCCCGCGAGTACGCCCAAGGCAAGCACTGTTCCACTGACGAACTCAAAGCCCGATTGAGCAGGAGGTTTGCC
CACAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E2L1N0

Antitoxin

Source ID Structure
AlphaFold DB A0A5Z3CIS0

References