Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 4188827..4189377 Replicon chromosome
Accession NZ_CP095086
Organism Escherichia coli isolate EC5654

Toxin (Protein)


Gene name ccdB Uniprot ID S1PDG4
Locus tag MUK77_RS20140 Protein ID WP_001160966.1
Coordinates 4188827..4189141 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag MUK77_RS20145 Protein ID WP_000125566.1
Coordinates 4189144..4189377 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MUK77_RS20115 (4184502) 4184502..4185788 + 1287 WP_000800457.1 peptidylprolyl isomerase SurA -
MUK77_RS20120 (4185788) 4185788..4186777 + 990 WP_000241229.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
MUK77_RS20125 (4186774) 4186774..4187595 + 822 WP_001065363.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
MUK77_RS20130 (4187598) 4187598..4187975 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
MUK77_RS20135 (4187982) 4187982..4188830 + 849 WP_000257182.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
MUK77_RS20140 (4188827) 4188827..4189141 - 315 WP_001160966.1 CcdB family protein Toxin
MUK77_RS20145 (4189144) 4189144..4189377 - 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
MUK77_RS20150 (4189463) 4189463..4189942 - 480 WP_000624375.1 type 3 dihydrofolate reductase -
MUK77_RS20155 (4190134) 4190134..4191996 - 1863 WP_000377124.1 glutathione-regulated potassium-efflux system protein KefC -
MUK77_RS20160 (4191989) 4191989..4192519 - 531 WP_000600725.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
MUK77_RS20165 (4192627) 4192627..4193958 - 1332 WP_001183207.1 MFS transporter -
MUK77_RS20170 (4194017) 4194017..4194304 - 288 WP_000203741.1 ferredoxin-like protein FixX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.72 Da        Isoelectric Point: 9.0851

>T241255 WP_001160966.1 NZ_CP095086:c4189141-4188827 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T241255 NZ_LT963408:547404-547754 [Pseudomonas syringae group genomosp. 3]
ATGAATACGTTTGCACTGCGCTTTACCGATGTTGCCCAGCAAAGCCTTGAGGATCAGGTTGAACACCTGGCTGTCCATCA
GGGATTCTCATCAGCTGCTCAACGCATAGATACCCTTATCGATGCCATCCAAGACAAGCTCCTGTCTACCCCGTTGGGTT
ACCCGGTCAGTCATCAATTGAGTGAGCTGGGTGTACTGCACTATCGTGAGCTGAACACTGACGGATACCGGATCTTTTAC
GAGGTGAGGGACGCAGGCGATATAAATGTCATCGTCGTGGCGCTCGTGTTAGGTAGCAAGCAAAGTGTTGAACAGGCCCT
CATCCGCTACTGCCTGCTTCAGGCTATATAG

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT241255 WP_000125566.1 NZ_CP095086:c4189377-4189144 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT241255 NZ_LT963408:547143-547391 [Pseudomonas syringae group genomosp. 3]
ATGCGAGTTGAAACAATCAGTTTTCTGAAACGCAACGCAGCCGACCTGCCTCTCGATGAGCCCTTGGTCGTCACGCAAAA
TGGTGTCCCGGCCTACGTGGTGGAGTCTTACGCTGATCGTAAGCGCCGCGATGAGTCAATCGCCTTGGTGAAGCTCCTGG
CAATCAGCTCCCGCGAGTACTCCCAAGGCAAGCACTGTTCCACTGACGAACTCAAAGCCCGATTGAGCAGGAGGTTTACC
CAGAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G815

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References