Detailed information of TA system TA241254

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pmenTA/darT(toxin)
• Location: 3919851..3921571
• Replicon: chromosome
• Accession: NZ_CP095086
• Organism: Escherichia coli isolate EC5654

Toxin (Protein)

• Gene name: pmenT
• Uniprot ID: A0A2T3TQG6
• Locus tag: MUK77_RS18950
• Protein ID: WP_024224217.1
• Coordinates: 3919851..3920507 (+)
• Length: 219 a.a.

Antitoxin (Protein)

• Gene name: pmenA
• Uniprot ID: -
• Locus tag: MUK77_RS18955
• Protein ID: WP_024224218.1
• Coordinates: 3920504..3921571 (+)
• Length: 356 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MUK77_RS18930 (3915162)	3915162..3915485	-	324	WP_157911636.1	hypothetical protein	-
MUK77_RS18935 (3915767)	3915767..3916006	+	240	WP_024224214.1	hypothetical protein	-
MUK77_RS18940 (3916046)	3916046..3918484	+	2439	WP_118253527.1	type I restriction-modification system subunit M	-
MUK77_RS18945 (3918484)	3918484..3919839	+	1356	WP_029786452.1	restriction endonuclease subunit S	-
MUK77_RS18950 (3919851)	3919851..3920507	+	657	WP_024224217.1	toxin-antitoxin system toxin DNA ADP-ribosyl transferase DarT	Toxin
MUK77_RS18955 (3920504)	3920504..3921571	+	1068	WP_024224218.1	toxin-antitoxin system antitoxin DNA ADP-ribosyl glycohydrolase DarG	Antitoxin
MUK77_RS18960 (3921590)	3921590..3924685	+	3096	WP_024224219.1	HsdR family type I site-specific deoxyribonuclease	-
MUK77_RS18965 (3924685)	3924685..3925401	+	717	WP_001122107.1	SprT family zinc-dependent metalloprotease	-
MUK77_RS25665 (3925623)	3925623..3925748	-	126	WP_021501707.1	hypothetical protein	-
MUK77_RS18970 (3925841)	3925841..3926215	-	375	WP_033815287.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fdeC / ykgK/ecpR / yagZ/ecpA / yagY/ecpB / yagX/ecpC / yagW/ecpD / yagV/ecpE / vat	3833468..3926215	92747

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ssDNA thymidine ADP-ribosyltransferase, DarT (17-217)

Antitoxin

• Macro domain (18-129)

Sequences

Toxin

Protein

• Length: 219 a.a.
• Molecular weight: 25232.57 Da
• Isoelectric Point: 6.4006

>T241254 WP_024224217.1 NZ_CP095086:3919851-3920507 [Escherichia coli]
MAYDYSASLNPQKALIWRIVHRDNIPWILDNGLHCGNSLVQAENWINIGNPELIGKRAGHPVPVGTGGTLHDYVPFYFTP
FSPMLMNIHSGRGGIKQRPNEEIVILVSNLRNVAAHDVPFVFTDSHAYYNWTNYYTSLNSLDQIDWPILQARDFRRDPDD
PAKFERYQAEALIWQHCPISLLDGIICYSEEVRLQLEQWLFQRNLTMSVHTRAGWYFS

Nucleotide

• Length: 657 bp

>T241254 NZ_LT963408:525533-526108 [Pseudomonas syringae group genomosp. 3]
ATGAGGCATTCGCCGTTTACAGCTTTCTATGACGCCAACGTCCTATACCCGGCACCGCTTCGTGACTTTCTGATGCACTT
GGCATTGACTGGCGTGTACCGCGCACGCTGGTCTTCTCAAATTCATGACGAGTGGAAGCGCAACCTTCTGATCAATCGTC
CAGAGCTTACCCGTGAGCAACTGGATCGTACGTCTGCGCTGATGGATAAGGCAGTGCCGGACGGTCTGGTATCTGATTAC
CAGTCGCTAATTGAAGGGCTTAAGCTGCCTGACGCCGATGATCGTCATGTGCTGGCGGCGGCAATCAAGTGCAATGCCTC
TGTCATCGTGACGTTCAACCTCAAGGACTTTCCGAAAGACGTCCTCGATACGTTCGATATGGAGCCCTTGCACCCGGATG
ATTTCATTGCCGACCTTTGGGATCTCGACAAAGCCGCTGTTCTCGAAGCCGCACAACGGCAGCGAGCATCTTTGAGAAAC
CCAGCACACAGTGCAAGGCAATACCTCGATAAGCTGTTGCAGCAGAAGCTTCCCGAGACTGTAAAACTGCTTTCGGATTT
TGAGTTTCTGATCTGA

Antitoxin

Protein

• Length: 356 a.a.
• Molecular weight: 40671.76 Da
• Isoelectric Point: 6.1751

>AT241254 WP_024224218.1 NZ_CP095086:3920504-3921571 [Escherichia coli]
MITYTQGNLLDAPVEALVNTVNTVGVMGKGIALMFKERFPENMKVYALACKQKQVITGKMFITETGELMGPRWIVNFPTK
QHWRADSRMEWIEDGLQDLRRFLIEENVQSIAIPPLGAGNGGLNWPDVRAQIESALGDLQDVDILIYQPTEKYQNVAKST
GVKKLTPARAAIAELVRRYWVLGMECSLLEIQKLAWLLQRAIEQHQQDDILKLRFEAHYYGPYAPNLNHLLNALDGTYLK
AEKRIPDSQPLDVIWFNDQKKEHVNAYLNNEAREWLPALEQVSQLIDGFESPFGLELLATVDWLLSRGECQPTLDSVKEG
LHQWPAGKRWASRKLRLFDNNNLQFAINRVMEFHC

Nucleotide

• Length: 1068 bp

>AT241254 NZ_LT963408:525069-525536 [Pseudomonas syringae group genomosp. 3]
ATGAGCAGCGCAGACGCCACTCGCACCGTTCTTCCTGGGGAGAAAGAAATCGCTGCGGCGGTTGAATCAAGTCGGCAATT
AGCCGCGTTTCTTACAACGAAATGTGACACCCAGCGCATTGAGCTTGTGGACGAAACGCAACAGCGCGAAGTCGTCGAGC
TGCCTATGTTTGCACTCCGTTTGCTCGGCGAAATTCTCAGTGAGCTTGCGCTGGGTAACGCCGTCAAGGTGGTCCCCATT
CATGCTGAACTCACTACTCAGGAAGCAGCAGACATGCTCAATGTGTCCCGGCCTCATCTGGTGAAGATGCTGGACCAAGG
CATGTTGCCGCACACCAAAACCGGCAGGCACCGCCGAGTCAAATTTGCTGATTTGATGAGCTACAAGCAGCAGCGCGACC
AGGCAAGTCGCGAAGCCATGGACGAGTTGGCCGCGCAAGCCCAAGAACTAGGGATGGGATATGAATGA

Structures

Toxin

• AlphaFold DB: A0A2T3TQG6