Detailed information of TA system TA241253

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3894551..3895385
• Replicon: chromosome
• Accession: NZ_CP095086
• Organism: Escherichia coli isolate EC5654

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: MUK77_RS18835
• Protein ID: WP_024224470.1
• Coordinates: 3894551..3894928 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: MUK77_RS18840
• Protein ID: WP_024224469.1
• Coordinates: 3895017..3895385 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MUK77_RS18805 (3890320)	3890320..3890478	+	159	WP_000550450.1	hypothetical protein	-
MUK77_RS18810 (3890545)	3890545..3890697	-	153	WP_000788776.1	hypothetical protein	-
MUK77_RS18815 (3891512)	3891512..3892831	+	1320	WP_000144688.1	site-specific integrase	-
MUK77_RS18820 (3892924)	3892924..3893772	-	849	WP_024224473.1	DUF4942 domain-containing protein	-
MUK77_RS18825 (3893857)	3893857..3894054	-	198	WP_024224472.1	DUF957 domain-containing protein	-
MUK77_RS18830 (3894066)	3894066..3894554	-	489	WP_024224471.1	DUF5983 family protein	-
MUK77_RS18835 (3894551)	3894551..3894928	-	378	WP_024224470.1	TA system toxin CbtA family protein	Toxin
MUK77_RS18840 (3895017)	3895017..3895385	-	369	WP_024224469.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
MUK77_RS18845 (3895464)	3895464..3895634	-	171	Protein_3703	DUF987 family protein	-
MUK77_RS18850 (3895720)	3895720..3896466	-	747	WP_001016257.1	IS21-like element ISEc12 family helper ATPase IstB	-
MUK77_RS18855 (3896481)	3896481..3898022	-	1542	WP_001620815.1	IS21-like element ISEc12 family transposase	-
MUK77_RS18860 (3898341)	3898341..3898817	-	477	WP_001380761.1	RadC family protein	-
MUK77_RS18865 (3898833)	3898833..3899306	-	474	WP_024224468.1	antirestriction protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fdeC / ykgK/ecpR / yagZ/ecpA / yagY/ecpB / yagX/ecpC / yagW/ecpD / yagV/ecpE / vat	3833468..3926215	92747

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13976.03 Da
• Isoelectric Point: 7.3580

>T241253 WP_024224470.1 NZ_CP095086:c3894928-3894551 [Escherichia coli]
MKTLPVLPGLAASSHPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAEAPSQLINSIDILRARRATGLMTRNNYRMVNNITQGKHPEAKR

Nucleotide

• Length: 378 bp

>T241253 NZ_LT963408:180170-180448 [Pseudomonas syringae group genomosp. 3]
ATGATCGTGAGTTTTAAGTGCGTGCACACGAGATATCTCTTTTTACAAGGCAAGACGCGGTTGTTGCCATCGATTAAATC
TGTTGCCGAGCGCAAGCTCGCAATGCTTGATGCTGCTACCTCGATTTTGGATCTGCGCTCTCCTCCCGGCAATCGACTAG
AGGCGCTTGATGGAAGTCGTTCTGGCCAATACAGCGTACGTATAAACGCTCAATTCAGGATTTGCTTCGTTTGGAGCATC
AACGGTCCCGAGGACGTAGAAATTGTCGATTATCACTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13625.47 Da
• Isoelectric Point: 6.4768

>AT241253 WP_024224469.1 NZ_CP095086:c3895385-3895017 [Escherichia coli]
VSDILPGTTHPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDVDAYHLDQAFPLLMKQLELMLTGG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT241253 NZ_LT963408:180458-180763 [Pseudomonas syringae group genomosp. 3]
ATGAACAAAAACGGAATGCGCCCCGTCCATCCAGGCGAGGTTCTGAAAGAGGAATATCTTGAGCCGATGGGGCTGACCGC
CGCAGCGTTGGCCAGGGCCCTTAAGGTTTCTACGCCGACGGTGAACGACATCGTGCTCCAGCGCAGAGGTGTGAGCGCTG
ATGTAGCGCTGCGTCTGGCTGTATGCCTGGAGACGAGTCCTGAGTTCTGGCTTAACCTGCAGCTTGCCTACGATCTGCGC
AAGGCAGAGACAGAGAAGGGCGCGCAGATTCGTGAGCAGGTCAAATGTCTGGCCCACTGCGCCTGA