Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3695033..3695651 Replicon chromosome
Accession NZ_CP095086
Organism Escherichia coli isolate EC5654

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag MUK77_RS17890 Protein ID WP_001291435.1
Coordinates 3695433..3695651 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag MUK77_RS17885 Protein ID WP_000344800.1
Coordinates 3695033..3695407 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MUK77_RS17875 (3690123) 3690123..3691316 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
MUK77_RS17880 (3691339) 3691339..3694488 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
MUK77_RS17885 (3695033) 3695033..3695407 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
MUK77_RS17890 (3695433) 3695433..3695651 + 219 WP_001291435.1 HHA domain-containing protein Toxin
MUK77_RS17895 (3695824) 3695824..3696375 + 552 WP_118253348.1 maltose O-acetyltransferase -
MUK77_RS17900 (3696491) 3696491..3696961 + 471 WP_000136192.1 YlaC family protein -
MUK77_RS17905 (3697125) 3697125..3698675 + 1551 WP_001468081.1 cyclic-guanylate-specific phosphodiesterase PdeB -
MUK77_RS17910 (3698717) 3698717..3699070 - 354 WP_000878142.1 DUF1428 family protein -
MUK77_RS17920 (3699449) 3699449..3699760 + 312 WP_000409908.1 MGMT family protein -
MUK77_RS17925 (3699791) 3699791..3700363 - 573 WP_000779844.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T241252 WP_001291435.1 NZ_CP095086:3695433-3695651 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T241252 NZ_LT963407:c2971-2552 [Pseudomonas syringae pv. avii]
ATGATCCTGCTCGATACGAACGTAATTTCAGAACCGCAGCGCCGAGAGCCCAATGCCCACGTCCTTGACTGGATCGACGC
ACAAGCGCTGGAAACGCTTTATCTGTCCACCATCACGGTAGCCGAGTTGCGTGCAGGTATCGCGCTGATGCCTGTCGGAA
AACGCCAGGATAGCTTGCGTGAAAACCTGGAAAAGCACCTGCTGCCGATGTTCGCCAATAGGGTGCTGTCGTTCGATATG
ACTTGTACGAAGACTTATGCCGAGCTGCTGGCCAAGTCCCGCGCAGCCGGGTTGGCGGTCGAAACGGCAGATGCCTTCAT
TGCAGCCATCGCCCTCGCCAACGGCTTCACCGTCGCCACCCGTGACACTGGCCCTTTTGAGGCTGCCGGGTTGAACGTCA
TTAACCCCTGGGAAGCGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT241252 WP_000344800.1 NZ_CP095086:3695033-3695407 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT241252 NZ_LT963407:c3225-2968 [Pseudomonas syringae pv. avii]
GTGGCAAACGTGAGCGTAAGAAATCTACCCGATGAAGTGCATCGGGCAATCCGCATCCAAGCTGCCCTGCACGGGCGCAG
CACCGAGGCCGAAATACGCGACATCTTGGAGCGGGCGGCGAGACCCGAGGGACGTGTGAAACTTGGCTCGTTCCTGGCTT
CTATTGCCCGTGAGGTTGGTGGTCTGACCGATAAAGAACACACCTTGTTTGAGAACACGCGCATCACGTCTCCGGCCCGT
GCGGTGAGCTTTGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References