Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 3004397..3005198 Replicon chromosome
Accession NZ_CP095086
Organism Escherichia coli isolate EC5654

Toxin (Protein)


Gene name yeeV Uniprot ID A0A0H2V687
Locus tag MUK77_RS14550 Protein ID WP_000854739.1
Coordinates 3004397..3004777 (-) Length 127 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A0P7R0L9
Locus tag MUK77_RS14555 Protein ID WP_001285482.1
Coordinates 3004824..3005198 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MUK77_RS14515 (2999989) 2999989..3000543 - 555 WP_001001921.1 molecular chaperone YcdY -
MUK77_RS14520 (3000567) 3000567..3001304 - 738 WP_000283664.1 zinc-binding phosphatase -
MUK77_RS14525 (3001359) 3001359..3002297 - 939 WP_000351277.1 glyoxylate/hydroxypyruvate reductase GhrA -
MUK77_RS14535 (3002767) 3002767..3003610 - 844 Protein_2859 DUF4942 domain-containing protein -
MUK77_RS14540 (3003695) 3003695..3003892 - 198 WP_000772027.1 DUF957 domain-containing protein -
MUK77_RS14545 (3003912) 3003912..3004400 - 489 WP_001518535.1 DUF5983 family protein -
MUK77_RS14550 (3004397) 3004397..3004777 - 381 WP_000854739.1 TA system toxin CbtA family protein Toxin
MUK77_RS14555 (3004824) 3004824..3005198 - 375 WP_001285482.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
MUK77_RS14560 (3005248) 3005248..3005892 - 645 WP_000086759.1 hypothetical protein -
MUK77_RS14565 (3005911) 3005911..3006132 - 222 WP_000692315.1 DUF987 domain-containing protein -
MUK77_RS14570 (3006195) 3006195..3006671 - 477 WP_044502179.1 RadC family protein -
MUK77_RS14575 (3006687) 3006687..3007160 - 474 WP_001313575.1 antirestriction protein -
MUK77_RS14580 (3007254) 3007254..3007499 - 246 WP_001164966.1 hypothetical protein -
MUK77_RS14585 (3007499) 3007499..3008320 - 822 WP_001535267.1 DUF932 domain-containing protein -
MUK77_RS14590 (3008499) 3008499..3008588 - 90 WP_112031555.1 DUF905 family protein -
MUK77_RS14595 (3008731) 3008731..3009186 - 456 WP_000581502.1 IrmA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 14214.14 Da        Isoelectric Point: 6.4773

>T241251 WP_000854739.1 NZ_CP095086:c3004777-3004397 [Escherichia coli]
MKTLPDTHVREASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGVPSQLINSIDILRARRATGLMTRDNYRTVNDITLGRHPEEAKQ

Download         Length: 381 bp

>T241251 NZ_LT963406:c60039-59740 [Pseudomonas syringae pv. avii]
ATGACGACGATAAAACAGACATCTACCTATATGGCTTGGGAACGCAGGCTCAAAGACCAAAAGGCCAAAGCTGCAATTGC
CGCTCGTATTTTTAGAGTTGCAAATGGCCTGATGGGTGATGTCTCGCCAGTAGGGCAGGGCGTGAGTGAGCTACGGATAC
ACGTTGGCCCTGGTTACCGTGTTTATTTCCAGCAGCGAGGCGATGAACTGATCTTGCTGCTGTGCGGTGGTGACAAGAGC
AGCCAATCCCGAGATATCGAAACAGCAAAGAGACTGGCTGATCAATGGAGGCAAGAATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 13791.44 Da        Isoelectric Point: 4.7511

>AT241251 WP_001285482.1 NZ_CP095086:c3005198-3004824 [Escherichia coli]
VSDTLHETNYPDDNNDRPWWGLPCTVTPCFGARLVQEGNQLHYLADRAGIRGQFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYARGLTCEADTLGSCGYVYMAVYPTLAPATTS

Download         Length: 375 bp

>AT241251 NZ_LT963406:c59743-59459 [Pseudomonas syringae pv. avii]
ATGAAAGAACAAATTTTCGATTACGACCCAGCAGCAGCCCTTGACGGGCCTGAGGCAATCGCTATTTTTATCTCTGACGC
ATTTGAGACGGGGGATGCGGAGTACATCGCGAAGGCTATGGGGGTTGTCGCAAGGGCCAAAGGGATGAGCGAGCTTGCGC
GTGAGACAGGTCTTTCTCGAGAGCAGCTTTACAGATCGTTCAGTAGCAAGGGCAATCCAACCTTAAAAACCATGTTGACG
GTAATGAGAGCCCTGGGCGTGGACATGACAGCTCGAATGCACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H2V687

Antitoxin

Source ID Structure
AlphaFold DB A0A0P7R0L9

References