Detailed information of TA system TA241249

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2540077..2541665
• Replicon: chromosome
• Accession: NZ_CP095086
• Organism: Escherichia coli isolate EC5654

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: B7MMY1
• Locus tag: MUK77_RS12100
• Protein ID: WP_001125475.1
• Coordinates: 2540343..2541665 (+)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: MUK77_RS12095
• Protein ID: WP_001332145.1
• Coordinates: 2540077..2540343 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MUK77_RS12080 (2536445)	2536445..2537896	+	1452	WP_000854636.1	tagaturonate reductase	-
MUK77_RS12085 (2538093)	2538093..2539007	+	915	WP_001306069.1	bestrophin family protein	-
MUK77_RS12090 (2539011)	2539011..2539769	-	759	WP_001286588.1	trans-aconitate 2-methyltransferase	-
MUK77_RS12095 (2540077)	2540077..2540343	+	267	WP_001332145.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
MUK77_RS12100 (2540343)	2540343..2541665	+	1323	WP_001125475.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
MUK77_RS12105 (2541924)	2541924..2542585	-	662	Protein_2377	alpha/beta hydrolase	-
MUK77_RS12110 (2542574)	2542574..2543053	+	480	WP_015912499.1	cupin domain-containing protein	-
MUK77_RS12115 (2543031)	2543031..2543792	+	762	WP_001066622.1	carboxymuconolactone decarboxylase family protein	-
MUK77_RS12120 (2543950)	2543950..2545014	+	1065	WP_000859696.1	alpha/beta fold hydrolase	-
MUK77_RS12125 (2545263)	2545263..2545735	-	473	Protein_2381	LysR substrate-binding domain-containing protein	-
MUK77_RS12130 (2545872)	2545872..2546654	+	783	WP_000699097.1	SDR family oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-401)

Antitoxin

• Cro/C1-type helix-turn-helix domain (18-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49426.24 Da
• Isoelectric Point: 8.9380

>T241249 WP_001125475.1 NZ_CP095086:2540343-2541665 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRRNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVMCPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGRVRALAVKRFDRRW
NTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIAQIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGIK

Nucleotide

• Length: 1323 bp

>T241249 NZ_LT963406:12662-12940 [Pseudomonas syringae pv. avii]
ATGCAAATCATCTGGCGGCAACGTGCGCGCATGAGCCTGGCTAAAATCATCCGGTATATCTCCAACGAAGACCCGCAAGC
CGCCCAAGCGATTCTAGAGCGTTTACAGTCGGCCATACTGCCGGTGGCCGATCATCCATACCTGTACCGCCCTGGACGTG
TGCCAGGCACCCGCGAGCTGGTGGCCCACCCGAACTACGTCCTGGTCTACCGCGTCACGCTTGAGCGCATCGAGGTCGTG
AACGTAATCCACGCTAGGCAGGAATACCCCTCGCTCTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10016.44 Da
• Isoelectric Point: 6.2267

>AT241249 WP_001332145.1 NZ_CP095086:2540077-2540343 [Escherichia coli]
MMSFQKIYSPMQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPNNTSLTTFFKILQSLELSMTLCDAKNASPEA
TEQQDLEW

Nucleotide

• Length: 267 bp

>AT241249 NZ_LT963406:12462-12662 [Pseudomonas syringae pv. avii]
ATGGCCAACCTCTCACCCATCGTTTCGGAATTCGAAACCGATGAACAAGCAGCAAGCTACGACCGTTGGTTTCGGCTCCA
GGTGCAAGCATCACTGGACGATCCCAGTCCAGGTGTGCCGCACGATCAGGTCATGGCGGAAATGGACGCAATCATTGCCG
AGGCAGAAAAGCGTCAACAGGATCGGACTAAGGTCAGCTGA

Structures

Toxin

• AlphaFold DB: A0A0E2L5M8