Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 1963784..1964615 Replicon chromosome
Accession NZ_CP095086
Organism Escherichia coli isolate EC5654

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag MUK77_RS09340 Protein ID WP_000854814.1
Coordinates 1963784..1964158 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID S1PWQ3
Locus tag MUK77_RS09345 Protein ID WP_001285586.1
Coordinates 1964247..1964615 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MUK77_RS09300 (1959181) 1959181..1960347 + 1167 WP_001297905.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -
MUK77_RS09305 (1960465) 1960465..1960938 + 474 WP_001105376.1 DNA gyrase inhibitor SbmC -
MUK77_RS09310 (1961136) 1961136..1962194 + 1059 WP_001200890.1 FUSC family protein -
MUK77_RS09315 (1962366) 1962366..1962695 + 330 WP_000450409.1 DUF496 family protein -
MUK77_RS09320 (1962796) 1962796..1963119 - 324 WP_223216378.1 EutP/PduV family microcompartment system protein -
MUK77_RS09325 (1963098) 1963098..1963178 + 81 WP_023441679.1 hypothetical protein -
MUK77_RS09330 (1963467) 1963467..1963547 - 81 Protein_1833 hypothetical protein -
MUK77_RS09335 (1963593) 1963593..1963787 - 195 WP_000988601.1 DUF5983 family protein -
MUK77_RS09340 (1963784) 1963784..1964158 - 375 WP_000854814.1 type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA Toxin
MUK77_RS09345 (1964247) 1964247..1964615 - 369 WP_001285586.1 type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA Antitoxin
MUK77_RS09350 (1964689) 1964689..1964910 - 222 WP_000692323.1 DUF987 domain-containing protein -
MUK77_RS09355 (1964979) 1964979..1965455 - 477 WP_001351157.1 RadC family protein -
MUK77_RS09360 (1965471) 1965471..1965956 - 486 WP_000213703.1 antirestriction protein -
MUK77_RS09365 (1966047) 1966047..1966868 - 822 WP_001234569.1 DUF932 domain-containing protein -
MUK77_RS09370 (1967089) 1967089..1967499 - 411 WP_000846704.1 hypothetical protein -
MUK77_RS09375 (1967515) 1967515..1968192 - 678 WP_001362823.1 hypothetical protein -
MUK77_RS09380 (1968328) 1968328..1969398 - 1071 WP_000102631.1 patatin-like phospholipase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - gtrB / gtrA 1952203..1986008 33805


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T241240 WP_000854814.1 NZ_CP095086:c1964158-1963784 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T241240 NZ_LT963402:3913350-3913631 [Pseudomonas syringae pv. avii]
ATGCAGGTTGAATGGCTAAAAACAGCATTAAAAAACCTGGATGATGAAGCCGCCTACATCTCGCTAGAAAATCCGGCTGC
TGCGGTTGCTTTCGTAGAAGCAATACAAATAAGCGTAAAGCAGCTCGCAAGCTTTCCGGCCCTAGGCCGAGAGGGACGAA
TTGCTGGCACCAGAGAATGGCCTTTGCCAGACTGGCCTTACCTGATTCCATACCGCATCCGTAACGGCCGATTACAGGTT
CTGCGGAATTTCCACACGAGAAGGCAGTCGCCGTTGGTGTAG

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13563.41 Da        Isoelectric Point: 5.0468

>AT241240 WP_001285586.1 NZ_CP095086:c1964615-1964247 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELSPRYQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT241240 NZ_LT963402:3913121-3913360 [Pseudomonas syringae pv. avii]
ATGTCCGTTATGTCTCTACGCCTGCCGGACGAGATGGCAGATACCCTTGCACACCTCGCCAAAGCGACCGGCCGCAGCAA
GTCGTTTTTGGCCTTGAACGCATTACGTGAATACTTGACTCGCGAAGCCTGGCAGATTGCCGAAATTCAGCGTGCAATTG
AAGAAGCAGACGCGGGCGAGTTCGCAAGTGAAGAAGATGTAAAGGCAGTGATGAATAAATGGGCGAACAATGCAGGTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
AlphaFold DB S1PWQ3

References