Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 980722..981376 Replicon chromosome
Accession NZ_CP095086
Organism Escherichia coli isolate EC5654

Toxin (Protein)


Gene name cptA Uniprot ID -
Locus tag MUK77_RS04830 Protein ID WP_016238471.1
Coordinates 980969..981376 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag MUK77_RS04825 Protein ID WP_000354046.1
Coordinates 980722..980988 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MUK77_RS04805 (976810) 976810..978243 - 1434 WP_001350137.1 6-phospho-beta-glucosidase BglA -
MUK77_RS04810 (978288) 978288..978599 + 312 WP_001182948.1 N(4)-acetylcytidine aminohydrolase -
MUK77_RS04815 (978763) 978763..979422 + 660 WP_000250274.1 hemolysin III family protein -
MUK77_RS04820 (979499) 979499..980479 - 981 WP_000886080.1 tRNA-modifying protein YgfZ -
MUK77_RS04825 (980722) 980722..980988 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
MUK77_RS04830 (980969) 980969..981376 + 408 WP_016238471.1 protein YgfX Toxin
MUK77_RS04835 (981416) 981416..981937 - 522 WP_001055874.1 flavodoxin FldB -
MUK77_RS04840 (982049) 982049..982945 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
MUK77_RS04845 (982970) 982970..983680 + 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
MUK77_RS04850 (983686) 983686..985419 + 1734 WP_000813190.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16018.96 Da        Isoelectric Point: 11.2669

>T241237 WP_016238471.1 NZ_CP095086:980969-981376 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEILLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T241237 NZ_LT963402:c271830-271558 [Pseudomonas syringae pv. avii]
GTGCTAGTTGAGTGGCTTCCACAAGCTTTGGAGGCACTGGAGGAAATCGCCGGCTACCTAGCCGAGCGCAACCCTTATGC
TGCCGAGCAGATGCAGGCAACCCTTGAGGCCATGGTCGAGGCTCTCCCCCTGCACCCTTACATCCACCGTCCCGGCCGTG
TTCCAGGAACGCGCGAAGCGGTTGTGCATCCCAACTACCTGGTTGTCTACCGCGTCGGTACAGAGCGCATCAGCATCTTG
GACGTCCTGCACGCCCGCCAAGAGTACCCCTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT241237 WP_000354046.1 NZ_CP095086:980722-980988 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT241237 NZ_LT963402:c272005-271820 [Pseudomonas syringae pv. avii]
ATGAGCATTCAACTTGACCCTCGCGTCTCGGAGTTCGAGACCCAAGAACAGGCCGACAATTACGACCGTTGGTTTCGCCA
GCGTATCGAGCACAGTTTGGCCGATCCGCGTCCACCCGTGCCGCACGATGAAGCAATGGCTCGCGTCAGAGCCATGATCG
AGGCAAAGCGCCGCCGTGCTAGTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References